product line
BioReagent
Quality Level
form
solid
solubility
DMF: soluble, DMSO: soluble, acetonitrile: soluble
fluorescence
λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
2-8°C
Application
BCECF 的细胞渗透性酯，通过胞质酯酶水解，产生胞内捕获的 pH 指示剂 BCECF。允许同时记录单个骨肉瘤细胞的细胞体积变化和细胞内 pH 值。
一种不带电的、无荧光的分子，对细胞悬液的非侵入性批量加载有帮助，因为它能透过细胞膜。细胞非特异性酯酶裂解其亲脂性阻断基团，产生带电荷的分子荧光形式。与母体化合物相比，这种带电荷的衍生物以慢得多的速率漏出电池。用于测定灌注组织、细胞间隙 、哺乳动物细胞 、植物细胞、细菌和酵母菌的 pH 值。此外，还用于研究细胞功能属性试验，如粘附、多药耐药性、活力和细胞毒性、细胞凋亡和趋化性。
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
商品
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
G R Martin et al.
Cancer research, 54(21), 5670-5674 (1994-11-01)
The tumor interstitial pH and its modification play a significant role in cancer treatment. Current in vivo pH measurement techniques are invasive and/or provide poor spatial resolution. Therefore, there are no data on perivascular interstitial pH gradients in normal or
A M Paradiso et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(23), 7436-7440 (1984-12-01)
We have used the pH-sensitive, fluorescent, cytoplasmic-trapped dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) to identify Na+-H+ exchange in gastric glands isolated from rabbit stomachs by high-pressure perfusion and collagenase digestion. The fluorescence of BCECF-loaded glands was calibrated in terms of cytosolic pH (pHc)
J R Sellers et al.
Journal of immunological methods, 172(2), 255-264 (1994-06-24)
A cytotoxicity assay has been developed based on the measurement of the proliferative activity of surviving cells as quantified by a cell-incorporated fluorescent dye, 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). The BCECF proliferative assay is fast (the results are obtained within 3-4 days depending
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 14562-1MG | 04061826678664 |