产品线
BioReagent
表单
solid
溶解性
DMF: soluble
DMSO: soluble
acetonitrile: soluble
荧光
λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)
适用性
suitable for fluorescence
储存温度
2-8°C
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应用
BCECF 的细胞渗透性酯，通过胞质酯酶水解，产生胞内捕获的 pH 指示剂 BCECF。允许同时记录单个骨肉瘤细胞的细胞体积变化和细胞内 pH 值。
一种不带电的、无荧光的分子，对细胞悬液的非侵入性批量加载有帮助，因为它能透过细胞膜。细胞非特异性酯酶裂解其亲脂性阻断基团，产生带电荷的分子荧光形式。与母体化合物相比，这种带电荷的衍生物以慢得多的速率漏出电池。用于测定灌注组织、细胞间隙 、哺乳动物细胞 、植物细胞、细菌和酵母菌的 pH 值。此外，还用于研究细胞功能属性试验，如粘附、多药耐药性、活力和细胞毒性、细胞凋亡和趋化性。
包装
无底玻璃瓶。内含物在插入的融合锥体内。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
I D Weiner et al.
The American journal of physiology, 256(5 Pt 2), F957-F964 (1989-05-01)
The compound 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is being extensively used to measure intracellular pH (pHi) in a variety of tissue preparations. Use of this dye in the isolated perfused rabbit cortical collecting tubule (CCT) revealed two previously unreported findings. Apical incubation via
C Dive et al.
Cytometry, 11(2), 244-252 (1990-01-01)
We describe an improved twin-probe multiparameter flow cytometric technique to examine cell membrane permeability. Ability to retain preloaded intracellular bis-carboxyethyl carboxy fluorescein (BCECF, green fluorescence) and to exclude extracellular propidium (red fluorescence) is measured, simultaneously with forward and right-angle scatter.
C B Brezden et al.
Cytometry, 17(4), 343-348 (1994-12-01)
Determination of intracellular calcium levels in Chinese hamster ovary (CHO) cells using the fluorescent calcium probe indo-1AM was hindered by the low level of accumulation of indo-1 in these cells. CHO cells are known to express basal levels of the
D Molenaar et al.
Biochimica et biophysica acta, 1115(1), 75-83 (1991-11-14)
The cytoplasmic pH of Lactococcus lactis was studied with the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5 (and-6)-carboxyfluorescein (BCECF). A novel method was applied for loading bacterial cells with BCECF, which consists of briefly treating a dense cell suspension with acid in the
M A Kolber et al.
Journal of immunological methods, 108(1-2), 255-264 (1988-04-06)
In order to utilize a newly available scanning microfluorimeter for lymphocyte-mediated cytotoxicity assays, a number of commercially available fluorescent dyes were compared for their suitability as target cell markers. One of them, bis-carboxyethyl-carboxyfluorescein (BCEFCF), was useful for assays with about
商品
荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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