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Merck
CN

14871

Sigma-Aldrich

BICINE

BioUltra, ≥99.5% (T)

别名:

N,N-双(2-羟乙基)甘氨酸

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关于此项目

经验公式（希尔记法）:
C6H13NO4
化学文摘社编号:
150-25-4
分子量:
163.17
Beilstein:
1769362
EC 号:
205-755-1
MDL编号:
MFCD00004295
UNSPSC代码:
12161700
PubChem化学物质编号:
57647225

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产品线

BioUltra

方案

≥99.5% (T)

表单

powder or crystals

杂质

insoluble matter, passes filter test

灼烧残渣

≤0.1%

缺失

≤0.1% loss on drying, 20 °C (HV)

pH值（酸碱度）

4.0-5.5 (25 °C, 1 M in H2O)

有效pH范围

7.6-9.0

pKa (25 °C)

8.3

溶解性

H2O: 1 M at 20 °C, clear, colorless

痕量阴离子

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤20 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

紫外吸收

λ: 260 nm Amax: 0.070
λ: 280 nm Amax: 0.040

SMILES字符串

OCCN(CCO)CC(O)=O

InChI

1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11)

InChI key

FSVCELGFZIQNCK-UHFFFAOYSA-N

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应用

用于低温生化工作的推荐缓冲液。用于制备稳定的血清鸟嘌呤酶测定底物溶液。通过等发色团法，使用葡聚糖凝胶® ，作为间隔区分离血浆蛋白。
BICINE can be used to study biological buffers and zwitterionic compounds. N,N-Bis(2-hydroxyethyl)glycine has been used in a study that characterized refined crystal structures of human Ca2+/Zn2+-binding S100A3 protein. N,N-Bis(2-hydroxyethyl)glycine has also been used in a study to inform F1 antigen assembly.

法律信息

Sephadex is a registered trademark of Cytiva

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCBJ8363V
BCBJ0139V
BCBH6666V
BCBG4337V
BCBG2057V

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Masaki Unno et al.
Journal of molecular biology, 408(3), 477-490 (2011-03-08)
S100A3, a member of the EF-hand-type Ca(2+)-binding S100 protein family, is unique in its exceptionally high cysteine content and Zn(2+) affinity. We produced human S100A3 protein and its mutants in insect cells using a baculovirus expression system. The purified wild-type
The myosin dimer: an intermediate in the self-assembly of the thick filament of vertebrate skeletal muscle.
J S Davis et al.
FEBS letters, 140(2), 293-297 (1982-04-19)
E Gopinath et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(9), 3041-3044 (1989-05-01)
The nature and rate of reduction of Hg2+ to Hg0 by 1,5-dihydro-3,(3-sulfopropyl)lumiflavin (FIH2) in buffered aqueous solutions (pH 4.7) is dependent on the ligation of Hg2+. In the presence of N,N-bis(2-hydroxyethyl)glycine or when ligated to ethylenediaminetetraacetic acid, the reduction is
Mohamed Taha
Annali di chimica, 94(12), 971-978 (2005-02-04)
The second stage dissociation constant pK2 of N,N-bis-(2-hydroxyethyl)glycine (bicine) has been determined in aqueous solution at different ionic strengths and different temperatures, using pH-metric technique. The thermodynamic quantities (deltaG(o), deltaH(o), and deltaS(o)) have been studied and discussed. Evaluation of the
B M Altura et al.
British journal of pharmacology, 69(2), 207-214 (1980-06-01)
1 In vitro studies were undertaken on rat aortic strips and portal vein segments in order to determine whether or not several commonly used artificial buffers, i.e., tris(hydroxymethyl) aminomethane (Tris), N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES), morpholine propanesulphonic acid (MOPS), N,N bis(2-hydroxyethyl) glycine
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