biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
SP66, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (226R-24), vial of 0.5 mL concentrate (226R-25), bottle of 1.0 mL predilute (226R-27), vial of 1.0 mL concentrate (226R-26), bottle of 7.0 mL predilute (226R-28)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
isotype
IgG1
control
tonsil
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
Gene Information
human ... BCL2(596)
General description
BCL2 is a protein associated with apoptosis regulation produced by the bcl-2 gene, located on chromosome 14q32. BCL2 is comprised of an alpha (239 amino acids) and beta chain. BCL2 (and thus BCL2 alpha chain) is found in mitochondrial and nuclear membranes and in the cytosol rather than the cell surface. In normal lymphoid tissue, BCL2 (and BCL2 alpha) antibody reacts with small B-lymphocytes in the mantle zone and many cells within the T-cell areas. Anti-BCL2 alpha has shown consistent negative reaction on reactive germinal centers and positive staining of neoplastic follicles in follicular lymphoma. This difference in staining pattern is not due to down regulation or decreased BCL2 mRNA, but largely to a post-translational mechanism with resultant decrease in protein levels. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymphoid lesions. Anti-BCL2 has been used as an indicator of minimal residual disease in the bone marrow of follicular lymphoma patients when staining is strong and uniform.
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Other Notes
BCL2 Positive Control Slides, Product No. 226S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Manual of Diagnostic Antibodies for Immunohistology, 25-27 (2003)
K Cooper et al.
The Journal of pathology, 182(3), 307-310 (1997-07-01)
Recent studies have shown bcl-2 to be regulated by p53. Other studies have suggested an inverse relationship between p53 and bcl-2 protein expression in breast and colonic cancers and in a variety of subtypes of non-Hodgkin's lymphoma. This study investigates
R Chetty et al.
Journal of clinical pathology, 48(11), 1035-1038 (1995-11-01)
To establish the role of immunohistochemistry (using a limited panel of antibodies) in detecting minimal involvement by follicular lymphoma in routinely processed bone marrow trephine specimens, which show no obvious morphological (light microscopic) evidence of lymphoma; to determine whether bcl-2
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