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Merck
CN

31149

脱氧核糖核酸,低分子量 来源于鲑鱼精子

别名:

鲑鱼精子dsDNA, DNA

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关于此项目

化学文摘社编号:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
309-566-6
NACRES:
NA.52
MDL number:
Biological source:
fish
Form:
lyophilized powder
Solubility:
50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
Storage temp.:
2-8°C
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biological source

fish

grade

Molecular Biology, puriss. p.a.

form

lyophilized powder

impurities

≤5% protein

solubility

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

absorbance ratio

A260/280 nm ~1.4

storage temp.

2-8°C

Application

从鲑鱼精子中提取的不同浓度的低分子量脱氧核糖核酸已用于:
  • 研究DNA与硅取代羟基磷灰石纳米晶体的结合。
  • 在简单的电化学DNA生物传感器中检测和评估UV-C辐射和活性氧造成的DNA损伤。
  • 作为合成生物点的前体。

Biochem/physiol Actions

在各种研究应用中,鲑鱼精子DNA常被用作阻断剂。 已知它可以通过减少非特异性结合和背景信号来提高多AP测定的特异性和敏感性。

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
低风险生物材料

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Eiji Sunami et al.
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer
J J Gao et al.
Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
Thiol modification of silicon-substituted hydroxyapatite nanocrystals facilitates fluorescent labelling and visualisation of cellular internalisation
Williams RL et al
Journal of Material Chemistry B: Materials for Biology and Medicine, 35, 4370-4378 (2013)
Andrea Hájková et al.
Bioelectrochemistry (Amsterdam, Netherlands), 116, 1-9 (2017-03-18)
A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual
V N Kondratova et al.
Biochemistry. Biokhimiia, 74(11), 1285-1288 (2009-11-18)
A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters

全球贸易项目编号

货号GTIN
31149-10G-F04061826681626
31149-50G-F04061826681633

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