InChI key
NXBZXYJXNIFCRJ-UHFFFAOYSA-M
InChI
1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
SMILES string
[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
form
powder or crystals
quality
according to May-Grünwald
εmax
≥1000 at 635-655 nm in methanol, ≥650 at 512-532 nm in methanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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General description
伊红和亚甲蓝染色的混合物，用于鉴别微生物培养基中培养和鉴定病原微生物，例如 干燥奈瑟球菌 、、 醋酸钙不动杆菌 。
Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.
Application
Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
The effect of concentration of dyes on differentiation of enteric bacteria on eosin-methylene-blue agar.
Journal of Bacteriology, 45 (5), 471-471 (1943)
Detection of carboxylic acid group in organic compounds with eosin, mercurochrome, and methylene blue.
Johar GS.
Fresenius' Zeitschrift fur Analytische Chemie, 260 (2), 122-123 (1972)
Oncosis is associated with exposure of phosphatidylserine residues on the outside layer of the plasma membrane: a reconsideration of the specificity of the annexin V/propidium iodide assay.
Lecoeur H
Cytometry, 44 (1), 65-72 (2001)
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