Quality Segment
assay
≥70.0% (degree of coupling)
solubility
DMF: 1 mg/mL, clear
fluorescence
λex 583 nm; λem 605.0 nm±10 nm in PBS, pH 7
storage temp.
−20°C
General description
Abberior STAR 580 is the latest development for STED microscopy with a fluorescent dye in the orange regime. The dye can be excited from 550 to 580 nm. Abberior STAR 580 can substitute dyes like Atto™ 590/594 or AlexaFluor 584/594. The dye can most effectively be depleted in STED microscopy at 700 to 780 nm via e.g. a Ti:Sa laser or IR diodes.
Abberior STAR 580 is the dye of choice for orange fluorescence. Moreover, the dye is particularly designed and tested for 2-color STED microscopy in combination with Abberior STAR 635 using 2 separate STED wavelength. This offers the great advantage of using 2 regular (not featuring a long Stokes-shift) dyes, which are in general more photostable. Please see our 2-color dye section
Key features
Absorption Maximum, λmax:: 587 nm (PBS, pH 7.4; water)
584 nm (MeOH, aq. ACN)
Extinction Coefficient, ε(λmax):85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.17 (PBS, pH 7.4; water)
Correction Factor, CF280 = ε280/εmax: 0.15 (MeOH, aq. ACN)
Fluorescence Maximum, λfl:607 nm (PBS, pH 7.4)
604 nm (MeOH, aq. ACN)
Recommended STED Wavelength, λSTED:700 - 780 nm
Fluorescence Quantum Yield, η:0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.5 ns (PBS, pH 7.4)
Abberior STAR 580 is the dye of choice for orange fluorescence. Moreover, the dye is particularly designed and tested for 2-color STED microscopy in combination with Abberior STAR 635 using 2 separate STED wavelength. This offers the great advantage of using 2 regular (not featuring a long Stokes-shift) dyes, which are in general more photostable. Please see our 2-color dye section
Key features
- Exceptionally bright orange fluorescent dye
- Ideal for STED microscopy at 700-775 nm
- 2-color labeling partner with STAR 635P for 2-color STED microscopy
Absorption Maximum, λmax:: 587 nm (PBS, pH 7.4; water)
584 nm (MeOH, aq. ACN)
Extinction Coefficient, ε(λmax):85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.17 (PBS, pH 7.4; water)
Correction Factor, CF280 = ε280/εmax: 0.15 (MeOH, aq. ACN)
Fluorescence Maximum, λfl:607 nm (PBS, pH 7.4)
604 nm (MeOH, aq. ACN)
Recommended STED Wavelength, λSTED:700 - 780 nm
Fluorescence Quantum Yield, η:0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.5 ns (PBS, pH 7.4)
Application
Anti-Mouse IgG-Abberior® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections. Abberior® STAR 580 conjugated with secondary antibody has been used for dual-color STED imaging of cultured hippocampal neurons.
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable