Quality Level
form
liquid
composition
bromophenol blue, 0.004% , DTT, 400 mM , glycerol, 20% , SDS, 4% , TRIS, 0.125 M
pH
~6.8
suitability
in accordance for electrophoresis test
storage temp.
−20°C
Application
Laemmli裂解液是一种无气味的裂解缓冲液,可制备用于SDS凝胶电泳的蛋白质样品。
signalword
Danger
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
存储类别
8B - Non-combustible corrosive hazardous materials
Sean R Gallagher
Current protocols in protein science, Chapter 10, Unit 10-Unit 10 (2012-04-04)
One-dimensional gel electrophoresis of proteins provides information about the molecular size, amount, and purity of a protein sample. Separated proteins can be recovered from polyacrylamide gels for subsequent characterization by a variety of secondary techniques, such as mass spectrometry to
Vincent Archambault et al.
Genes & development, 22(19), 2707-2720 (2008-10-04)
The conserved Polo kinase controls multiple events in mitosis and cytokinesis. Although Polo-like kinases are regulated by phosphorylation and proteolysis, control of subcellular localization plays a major role in coordinating their mitotic functions. This is achieved largely by the Polo-Box
Xiaoqiang Wu et al.
Analytical biochemistry, 421(1), 347-349 (2011-11-01)
A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can
Sean R Gallagher
Current protocols in immunology, Chapter 8, Unit 8-Unit 8 (2008-04-25)
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can also serve to purify proteins for use in further
Lisha Mou et al.
PloS one, 10(7), e0133997-e0133997 (2015-07-25)
Secretory azoospermia is a severe form of male infertility caused by unknown factors. DAX-1 is predominantly expressed in mammalian reproductive tissues and plays an important role in spermatogenesis because Dax-1 knockout male mice show spermatogenesis defects. To examine whether DAX-1
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 38733-5ML | 04061838047588 |
| 38733-2ML | 04061838236128 |
| 38733-5X2ML | 04061838084361 |
| 38733-1ML | 04061838253583 |
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