biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
EP184, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (408R-14)
vial of 0.5 mL concentrate (408R-15)
bottle of 1.0 mL predilute (408R-17)
vial of 1.0 mL concentrate (408R-16)
bottle of 7.0 mL predilute (408R-18)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
isotype
IgG
control
kidney, renal oncocytoma
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic, nuclear
Gene Information
human ... S100A1(6271)
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
 IVD |  IVD |  IVD |  RUO |
General description
S100A1 is a member of S100 protein family of calcium binding proteins.S100A1 is very useful in the identification of renal cell carcinoma.Published literature indicates that 37 of 40 renal oncocytomas (93%), including two with the tubulo-cystic growth pattern, reacted positively by anti-S100A1.Most of them showed both cytoplasmic and nuclear immunostaining with moderate-to-strong intensity. S100A1 immunostaining was also observed in 30 of 41 clear cell renal cell carcinomas (73%); 6 showed focal positivity, whereas 24 displayed an immunoreactivity ranging from moderate (12 cases) to diffuse (12 cases) expression. Anti-S100A1 detected 30 cases of 32 papillary renal cell carcinomas (94%), 6 showing focal, 12 moderate, and 12 diffuse immunoreactivity. This study also demonstrated that 48 of the 51 chromophobe renal cell carcinomas (94%) showed no labeling by anti-S100A1. In 3 cases of chromophobe renal cell carcinomas (6%) that were positive by anti-S100A1, 2 were classic variant showing scattered S100A1 immunoreactive cells and an eosinophilic variant with nucleocytoplasmic staining in about 40% of the neoplastic cells.Therefore, anti-S100A1 is very useful in the differentiation of renal oncocytoma from chromophobe renal cell carcinoma as well as distinguishing clear cell renal cell carcinoma from chromophobe renal cell carcinoma. S100A1 is detected in normal renal tissue and expressed in both the nuclei and the cytoplasm of the cells lining proximal tubules, loops of Henle, and collecting ducts. The glomerular components are negative. S100A1 is also expressed by skeletal muscle and myocardium; and specific cytoplasmic expression is found in follicular dendritic cells of lymph node.
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
S100A1 Positive Control Slides, Product No. 408S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Paolo Cossu Rocca et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 20(7), 722-728 (2007-05-08)
S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas
D B Zimmer et al.
Brain research bulletin, 37(4), 417-429 (1995-01-01)
The S100 family of calcium binding proteins contains approximately 16 members each of which exhibits a unique pattern of tissue/cell type specific expression. Although the distribution of these proteins is not restricted to the nervous system, the implication of several
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