Anti-Rabbit IgG−Abberior^® STAR 580 antibody produced in goat

Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 580 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation wavelength between 550 - 600 nm. Abberior STAR 580 can substitute dyes like Cy3^™, Atto^™ 590 or Alexa Fluor^™ 568. For STED microscopy, Abberior STAR 580 can be used most efficiently with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 580 is the ideal partner for Abberior STAR RED or Abberior STAR 635P to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 587 (PBS pH 7.4), 584 (H₂O), 584 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 85 000 (PBS pH 7.4), 85 000 (H₂O), 95 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,17 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 607 (PBS pH 7.4), 607 (H₂O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)

Designed and tested for fluorescent super-resolution microscopy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy3 is a trademark of Cytiva

abberior is a registered trademark of Abberior GmbH