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Merck
CN

41785

溴化二亚胺

BioReagent, suitable for fluorescence, ~95% (AT)

别名:

3,8-二氨基-5-甲基-6-苯基菲啶鎓溴化物, 胰蛋白酶, 菲啶盐1553

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关于此项目

经验公式（希尔记法）:
C20H18BrN3
化学文摘社编号:
518-67-2
分子量:
380.28
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57650090
EC Number:
208-256-7
Beilstein/REAXYS Number:
3809779
MDL number:
MFCD00011757
Assay:
~95% (AT)
Form:
solid

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产品名称

溴化二亚胺, BioReagent, suitable for fluorescence, ~95% (AT)

InChI key

MQOKYEROIFEEBH-UHFFFAOYSA-N

InChI

1S/C20H17N3.BrH/c1-23-19-12-15(22)8-10-17(19)16-9-7-14(21)11-18(16)20(23)13-5-3-2-4-6-13;/h2-12,22H,21H2,1H3;1H

SMILES string

[Br-].C[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

product line

BioReagent

assay

~95% (AT)

form

solid

Quality Level

100

mol wt

380.28 g/mol

concentration

80-100 % (w/w) (3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide)

color

dark purple to very dark purple
dark red to very dark red

mp

243-248 °C (lit.)

fluorescence

λex 306 nm; λem 612 nm in 50 mM Tris pH 8.0 (DNA)
λex 480 (pH 7.6, low ionic strength)

suitability

suitable for fluorescence

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相关类别

Application

基于碱基对的特异性，溴甲菲啶被用作天然DNA的粘合剂。它在核酸实验中用作荧光剂。
核酸插入探针。

General description

溴甲菲啶是一种抗锥虫药物，已知它可在许多生物体中影响核酸合成，比如组织培养的细胞。

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN1632
BCCL9621
BCCJ8750
BCCL0032
BCCH5955

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Nuclear transplantation studies of the action of dimidium bromide on amoebae.
S E Hawkins et al.
Nature, 222(5188), 86-87 (1969-04-05)
G Dougherty
The International journal of biochemistry, 14(6), 493-504 (1982-01-01)
1. The absorption spectrum of three phenanthridine drugs (ethidium, dimidium and prothidium bromide) bound to natural DNAs of differing G-C content were obtained using a novel mixing scheme and analysed according to the excluded site binding model. 2. Ethidium bromide
Dimidium bromide or phenanthridinium 1553; a note on the present position.
J CARMICHAEL
The Veterinary record, 62(17), 257-257 (1950-04-29)
[Chemoprophylaxis of bovine trypanosomiasis T. congolense and T. vivax by phenathridinium S. 1553].
J DEOM
Annales de la Societe belge de medecine tropicale (1920), 30(1), 25-29 (1950-03-01)
T N Beliaeva et al.
Tsitologiia, 31(11), 1363-1368 (1989-11-01)
A natural DNA-intercalator plant benzo-c-phenanthridine alkaloid sanguinarine is more toxic for mouse transformed fibroblast L-cells in culture than synthetic DNA-intercalator ethidium bromide (EtB) and alkaloid berberine. Dimidium bromide is also an inhibitor of the L-cell growth. In assay conditions, growth
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Nancy-520 for DNA Detection and Quantitation

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