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Merck
CN

45125

Sigma-Aldrich

弹性蛋白酶 来源于猪胰腺

lyophilized, powder, white, ~8 U/mg

别名:

弹性蛋白酶 来源于猪胰腺, 胰酞酶 E

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UNSPSC代码:
12352204
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生物来源

Porcine pancreas

质量水平

表单

powder

质量

lyophilized

比活

~8 U/mg

分子量

Mr ~25000

杂质

salt, none detected

颜色

white

储存温度

−20°C

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生化/生理作用

弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。

其他说明

一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。
1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)
For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds
Sales restrictions may apply

法规信息

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分析证书(COA)

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I Grunnet et al.
The Biochemical journal, 209(1), 215-222 (1983-01-01)
Fatty acid synthetase from goat mammary gland was subjected to limited proteolysis by trypsin and elastase. Both proteolytic enzymes selectively cleaved the chain-terminating thioester hydrolase component from the enzyme complex, leaving all other partial activities intact in the core peptides.
T. Sakurai et al.
Journal of the American Chemical Society, 110, 7236-7236 (1988)
Commentary on 'Performance of a modified rabbit model of abdominal aortic aneurysm induced by topical application of porcine elastase: 5-month follow-up study'.
G W Cockerill
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, 45(2), 153-153 (2013-01-02)
Lilibeth A Salvador et al.
Journal of medicinal chemistry, 56(3), 1276-1290 (2013-01-29)
We discovered new structural diversity to a prevalent, yet medicinally underappreciated, cyanobacterial protease inhibitor scaffold and undertook comprehensive protease profiling to reveal potent and selective elastase inhibition. Structure-activity relationship (SAR) studies and X-ray cocrystal structure analysis allowed a detailed assessment
Brian P O'Sullivan et al.
The Journal of pediatrics, 162(4), 808-812 (2012-12-19)
To describe pancreatic function during the first year of life in infants diagnosed with cystic fibrosis (CF) using serial fecal elastase measurements. This was a longitudinal study of 82 infants diagnosed with CF through newborn screening. Monthly stool samples were

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