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Merck
CN

45125

Sigma-Aldrich

弹性蛋白酶 来源于猪胰腺

lyophilized, powder, white, ~8 U/mg

别名:

弹性蛋白酶 来源于猪胰腺, 胰酞酶 E

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UNSPSC代码:
12352204
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生物来源

Porcine pancreas

质量水平

表单

powder

质量

lyophilized

比活

~8 U/mg

分子量

Mr ~25000

杂质

salt, none detected

颜色

white

储存温度

−20°C

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生化/生理作用

弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。

其他说明

一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。
1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)
For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds
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分析证书(COA)

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I Grunnet et al.
The Biochemical journal, 209(1), 215-222 (1983-01-01)
Fatty acid synthetase from goat mammary gland was subjected to limited proteolysis by trypsin and elastase. Both proteolytic enzymes selectively cleaved the chain-terminating thioester hydrolase component from the enzyme complex, leaving all other partial activities intact in the core peptides.
T. Sakurai et al.
Journal of the American Chemical Society, 110, 7236-7236 (1988)
Commentary on 'Performance of a modified rabbit model of abdominal aortic aneurysm induced by topical application of porcine elastase: 5-month follow-up study'.
G W Cockerill
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, 45(2), 153-153 (2013-01-02)
Charles R Esther et al.
American journal of physiology. Lung cellular and molecular physiology, 304(7), L504-L509 (2013-01-29)
Measurement of exhaled breath condensate (EBC) biomarkers offers a noninvasive means to assess airway disease, but the ability of EBC biomarkers to track longitudinal changes in disease severity remains unproven. EBC was collected from pediatric patients with cystic fibrosis (CF)
Christine Terryn et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 83(2), 235-241 (2012-12-06)
The spatial organization of proteins in a cell population or in tissues is an important parameter to study the functionality of biological specimens. In this article, we have focused on tight junctions which form network-like features in immunofluorescence microscopy images.

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