46058
酯酶 来源于猪肝脏
lyophilized, powder, slightly beige, ≥50 U/mg
别名:
PLE, 羧酸酯水解酶, 羧酸酯酶
Application
从猪肝中提取的酯酶用作荧光测定的阴性对照。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成 。
猪肝酯酶被用于制药和工业应用中以催化五乙酰儿茶素和表儿茶素的水解。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成。
猪肝酯酶常用于有机化学中的动力学拆分和不对称合成。
Biochem/physiol Actions
猪肝酯酶（PLE）具有良好的稳定性和广泛的底物特异性，是一种低成本酶。它在酯类和含酰胺化合物的水解、游离酸和生物异源物质的解毒等发挥作用。
酯酶作用于含有短链脂肪酸的水溶性羧酸酯。其功能来自于Ser、His和Asp/Glu的催化三联体。
Disclaimer
可能适用相应的销售限制。
General description
猪肝酯酶（PLE）位于内质网（ER）中。
Other Notes
1U 对应于在 pH8.0 和 25°C 下每分钟水解 1μ 戊酸乙酯（目录号：30784）的酶量。
含 1,4-二硫赤藓糖醇
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
低风险生物材料
此项目有
Elke Brüsehaber et al.
Bioorganic & medicinal chemistry, 17(23), 7878-7883 (2009-11-04)
The possible physiological role of PLE (E.C. 3.1.1.1) located in the endoplasmic reticulum (ER) of pig liver cells in the conversion of endogenous compounds was investigated as it was reported, that PLE acts as prenylated methylated protein methyl esterase (PMPMEase)
Scale-up of a recombinant pig liver esterase-catalyzed desymmetrization of Dimethyl Cyclohex-4-ene-cis-1, 2-dicarboxylate
Su?ss P, et al.
Organic Process Research & Development, 18 (2014)
Active-site model for interpreting and predicting the specificity of pig liver esterase
Toone, EJ et al.
Journal of the American Chemical Society, 112, 4946-4952 (1990)
Qing Zhu et al.
Macromolecular rapid communications, 31(12), 1060-1064 (2011-05-19)
Herein we describe a novel and simple conjugated polymer-fluorescent probe based platform for trypsin detection from protein mixtures in homogeneous solution. This platform takes advantage of specific interaction between the probe and the active site of trypsin and the electrostatic
Bornscheuer, UT & Kazlauskas, RJ
Hydrolases in Organic Synthesis, 2 (2005)
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