47140
4-氟-7-硝基苯并-2-氧杂-1,3-二唑
suitable for fluorescence, BioReagent, ≥98.0% (HPLC)
别名:
4-氟-7-硝基-2,1,3-苯并氧杂恶二唑
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关于此项目
经验公式（希尔记法）:
C6H2FN3O3
化学文摘社编号:
分子量:
183.10
Beilstein:
1077571
MDL编号:
UNSPSC代码:
12352100
PubChem化学物质编号:
NACRES:
NA.32
产品线
BioReagent
方案
≥98.0% (HPLC)
mp
52-54 °C (lit.)
荧光
λex 470 nm; λem 528 nm in ethanol
λex 470 nm; λem 550 nm (Amino acid adducts)
适用性
suitable for fluorescence
储存温度
2-8°C
SMILES字符串
[O-][N+](=O)c1ccc(F)c2nonc12
InChI
1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
InChI key
PGZIDERTDJHJFY-UHFFFAOYSA-N
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应用
在高效液相色谱中可用于氨基酸和胺的荧光标记
4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
包装
Bottomless glass bottle. Contents are inside inserted fused cone.
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
235.4 °F - closed cup
闪点(°C)
113 °C - closed cup
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Hongyi Zhang et al.
Analytical and bioanalytical chemistry, 386(5), 1387-1394 (2006-09-06)
An in-capillary derivatization of amino acids and peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed for their subsequent capillary electrophoretic analysis with laser-induced fluorescence detection (lambda (ex)=488 nm). The in-capillary derivatization was achieved in zone-passing mode by introducing successive plugs of sample
Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm
Hai Han et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(29), 3196-3202 (2011-03-05)
For a metabolomics study focusing on the analysis of aspartic and glutamic acid enantiomers, a fully automated two-dimensional HPLC system employing a microbore-ODS column and a narrowbore-enantioselective column was developed. By using this system, a detailed distribution of D-Asp and
Chanda Ciriacks Klinker et al.
Analytical chemistry, 79(22), 8747-8754 (2007-10-13)
4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) has been characterized as a fluorogenic labeling reagent for high speed, online microdialysis-capillary electrophoresis (MD-CE) assays for amino acid neurotransmitters. NBD-F labeled amines are efficiently excited using 488 nm light allowing more common lasers to be used for
Bryan R Fonslow et al.
Analytical chemistry, 80(9), 3182-3189 (2008-03-21)
The continuous nature of micro free-flow electrophoresis (mu-FFE) was used to monitor the effect of a gradient of buffer conditions on the separation. This unique application has great potential for fast optimization of separation conditions and estimation of equilibrium constants.
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