Merck
CN
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文件

47671

Sigma-Aldrich

甲酰胺

BioUltra, for molecular biology, ≥99.5% (T)

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别名:
C1 酰胺, 氨基甲醛
线性分子式:
HCONH2
CAS号:
75-12-7
分子量:
45.04
Beilstein:
505995
EC 号:
200-842-0
MDL编号:
MFCD00007941
PubChem化学物质编号:
57651075
NACRES:
NA.25

等级

for molecular biology

蒸汽密度

1.55 (vs air)

蒸汽压

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

产品线

BioUltra

检测方案

≥99.5% (T)

形式

liquid

自燃温度

932 °F

technique(s)

hybridization: suitable

杂质

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

灼烧残渣

≤0.1%

折射率

n20/D 1.447 (lit.)
n20/D 1.447

pH值（酸碱度）

7-9 (25 °C, 1 M in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

溶解性

H2O: 10 M at 20 °C, clear, colorless

密度

1.134 g/mL at 25 °C (lit.)

痕量阴离子

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

痕量阳离子

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES string

NC=O

λ

10 M in H2O

紫外吸收

λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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相关类别

一般描述

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

应用

甲酰胺已用于：
  • 作为用于原位杂交的杂交缓冲液中的组分，作为溶液 X 的组分，用于洗涤载玻片以去除盖玻片
  • 光谱核型分析（SKY）的变性溶液的制备
  • 比较基因组杂交（CGH）载玻片预处理的 1% 甲醛溶液的制备
  • RNA 荧光原位杂交（RNA-FISH）的杂交前缓冲液 I、II、III、IV 和杂交缓冲液 I、II 和 III 的制备
  • 作为生产甲酸酯和维生素的电离溶剂，纸张的软化剂，动物胶和水溶性胶，分子生物学中的变性剂

甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

生化/生理作用

甲酰胺是极性有机和无机化合物的良好溶剂，由于其高介电常数，在益生元过程中具有潜在的催化活性。

特点和优势

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

其他说明

For additional information on our range of Biochemicals, please complete this form.
甲酰胺变性聚丙烯酰胺胶制备；人染色体原位杂交

象形图

Health hazard
GHS08

警示用语：

Danger

危险分类

Carc. 2 - Repr. 1B - STOT RE 2 Oral

靶器官

Blood

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

闪点(°F)

305.6 °F

闪点(°C)

152 °C

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the best way to deionize Formamide, Product 47671?

    Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.

  6. Are aqueous solutions of Formamide, Product 47671, stable?

    In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).

  7. What is the molarity of the neat liquid Formamide, Product 47671?

    Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
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