biological source
mouse
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
MRQ-69, monoclonal
description
For In Vitro Diagnostic Use in Select Regions
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (481M-94)
vial of 0.1 mL concentrate Research Use Only (481M-94-RUO)
vial of 0.5 mL concentrate (481M-95)
vial of 1.0 mL concentrate (481M-96)
vial of 1.0 mL concentrate Research Use Only (481M-96-RUO)
vial of 1.0 mL pre-dilute Research Use Only (481M-97-RUO)
vial of 1.0 mL pre-dilute ready-to-use (481M-97)
vial of 7.0 mL pre-dilute ready-to-use (481M-98)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (481M-98-RUO)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:200 (concentrated)
isotype
IgG
control
Ewing’s sarcoma, rhabdomyosarcoma
shipped in
wet ice
storage temp.
2-8°C
visualization
nuclear
Analysis Note
United States - IVD
Canada - RUO
European Union - IVD
Japan - RUO
Canada - RUO
European Union - IVD
Japan - RUO
General description
The paired-box (PAX) family of proteins are key transcriptional regulators involved in early critical development.1 The PAX-7 transcription factor has important functions in mammalian myogenesis and early neural development, with a particularly crucial role in specification and self-renewal of skeletal muscle tissue.2,3 The expression of PAX-7 is highly restricted in normal adult tissues as demonstrated by nuclear immunoreactivity only being identified in rare, scattered satellite cells of the skeletal muscle and absent in both visceral smooth muscle and cardiac muscle as well as in a broad anatomical range of other non-neoplastic tissues. The assessment of soft tissue tumors and small round cell tumors is a persistent diagnostic challenge because of overlapping morphological features and insufficient molecular characterization in these groups of neoplasms. A high frequency of strong PAX-7 nuclear expression has been identified predominantly in rhabdomyosarcomas, preferentially in the embryonal subtype, and Ewing′s sarcoma, with reactivity being absent in related malignancies such as leiomyosarcoma, lymphoblastic lymphoma, neuroblastoma, carcinoid tumor, gastrointestinal stromal tumor, and small cell lung carcinoma. Immunohistochemical detection of PAX-7 protein can be used as a tool in distinguishing embryonal rhabdomyosarcoma and Ewing′s sarcoma from histologic mimics.4,5
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.
Preparation Note
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Note: This IFU may not apply to your specific product lot.
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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Shunichi Toki et al.
Histopathology, 73(4), 645-652 (2018-06-20)
Ewing sarcoma is a small round cell tumour that affects bone and soft tissues. Although the detection of the specific fusion gene is a robust method of its diagnosis, immunohistochemistry may serve as a practical surrogate. Recent tissue microarray studies
Gregory W Charville et al.
The American journal of surgical pathology, 40(10), 1305-1315 (2016-08-16)
Rhabdomyosarcoma, the most common soft tissue malignancy of childhood, is a morphologically variable tumor defined by its phenotype of skeletal muscle differentiation. The diagnosis of rhabdomyosarcoma often relies in part on the identification of myogenic gene expression using immunohistochemical or
A Kawakami et al.
Mechanisms of development, 66(1-2), 119-130 (1997-08-01)
We describe here the isolation of chicken PAX6 and PAX7 as members of the PAX gene family expressed in late stages of chick nervous system development. By generating monoclonal antibodies against these PAX molecules, we analyzed their protein distributions in
Hugo C Olguin et al.
Developmental biology, 275(2), 375-388 (2004-10-27)
Satellite cells are myogenic precursors responsible for skeletal muscle regeneration. Satellite cells are absent in the Pax-7-/- mouse, suggesting that this transcription factor is crucial for satellite cell specification [Seale, P., Sabourin, L.A., Girgis-Gabardo, A., Mansouri, A., Gruss, P., Rudnicki
Judith A Blake et al.
Development (Cambridge, England), 141(4), 737-751 (2014-02-06)
Pax genes encode a family of transcription factors that orchestrate complex processes of lineage determination in the developing embryo. Their key role is to specify and maintain progenitor cells through use of complex molecular mechanisms such as alternate RNA splice
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