48275
β 半乳糖苷酶 来源于大肠杆菌
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
表单
powder
质量水平
质量
lyophilized
比活
~140 U/mg
分子量
Mr ~135000
包含
phosphate buffer as stabilizer
sucrose as stabilizer
技术
enzyme immunoassay: suitable
运输
wet ice
储存温度
−20°C
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一般描述
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
应用
水溶液可以直接用于衍生化，而无需事先进行透析或凝胶渗透色谱法。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
包装
无底玻璃瓶。内含物装在插入的融合锥内。
其他说明
1U对应于在pH 7.8和37℃下每分钟水解1 μmol 2-硝基苯基-β-D-吡喃半乳糖苷（目录号73660）所需的酶量。
用于4-甲基伞形酮基-β-D-半乳糖苷（目录号69590）参与的高灵敏度酶联免疫测定人IgG；夹心酶联免疫分析评价β-D-半乳糖苷酶（大肠埃希菌）和辣根过氧化物酶标记物；促甲状腺素释放激素（TRH）的酶联免疫测定；酶-抗体偶联物的制备
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
A Hinek et al.
The Journal of clinical investigation, 91(3), 1198-1205 (1993-03-01)
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for cell adhesion to elastin and laminin and for mediating the process of elastin fiber assembly, but the nature of this protein was unknown.
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