biological source
goat
recombinant
expressed in HEK 293 cells
conjugate
Atto 655 conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
contains
50% glycerol
species reactivity
mouse
concentration
~1 mg/mL IgG
technique(s)
immunofluorescence: suitable
fluorescence
λex 655 nm; λem 677 nm in PBS
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Anti-mouse IgG (whole molecule) (Sigma, M8645) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 655 NHS (Abs.max. 663 nm; Em.max. 684 nm),(Cat. No. 76245), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.
Immunogen
purified mouse IgG
Application
Atto 655-goat anti-Mouse IgG - Atto 655 antibody has been used for immunofluorescence applications.
Packaging
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Analysis Note
Unconjugated dye ≤5% of total fluorescence.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
pcodes
Hazard Classifications
Eye Irrit. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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