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Merck
CN

50980

异硫氰酸胍

BioUltra, Molecular Biology, ≥99.0% (AT)

别名:

硫氰酸亚脲, 硫氰酸胍

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关于此项目

线性分子式:
NH2C(=NH)NH2 · HSCN
化学文摘社编号:
分子量:
118.16
UNSPSC Code:
12352107
NACRES:
NA.25
PubChem Substance ID:
EC Number:
209-812-1
Beilstein/REAXYS Number:
3563461
MDL number:
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产品名称

异硫氰酸胍, BioUltra, Molecular Biology, ≥99.0% (AT)

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

SMILES string

SC#N.NC(N)=N

grade

Molecular Biology

product line

BioUltra

assay

≥99.0% (AT)

form

solid

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.5% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

4.5-7.0 (25 °C, 6 M in H2O)

mp

117-120 °C
120-122 °C (lit.)

solubility

H2O: 6 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5000 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

absorption

cut-off at 280 nm in H2O at 6 M

Quality Level

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Application

离液剂和强效变性剂;溶解细胞。

Other Notes

RNA的分离;一种新的硫氰酸胍应用方法,从富含核糖核酸酶和蛋白聚糖的组织中提取全长RNA模板进行逆转录

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

存储类别

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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J C Groppe et al.
Analytical biochemistry, 210(2), 337-343 (1993-05-01)
RNA isolated by conventional guanidinium isothiocyanate methods from tissues of a mollusc (red abalone: Haliotis rufescens) is largely degraded and discolored by contaminants. These contaminants are associated with inhibition of reverse transcriptase, prevent accurate spectrophotometric determination of RNA concentration, and
Turpen, T.H. and Griffith, O.M.
Biotechniques, 4, 11-11 (1986)
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is
Kai Y Mak et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1434-1443 (2015-05-23)
Angiotensin converting enzyme 2 (ACE2) which breaks down profibrotic peptide angiotensin II to antifibrotic peptide angiotensin-(1-7) is a potential therapeutic target in liver fibrosis. We therefore investigated the long-term therapeutic effect of recombinant ACE2 using a liver-specific adeno-associated viral genome
Yuji Suzuki et al.
Bioscience, biotechnology, and biochemistry, 72(7), 1951-1953 (2008-07-08)
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds

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