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Merck
CN

50983

异硫氰酸胍水溶液 溶液

BioUltra, Molecular Biology, ~6 M in H2O

别名:

异硫氰酸胍, 硫氰酸亚脲, 硫氰酸胍

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关于此项目

线性分子式:
NH2C(=NH)NH2 · HSCN
化学文摘社编号:
分子量:
118.16
UNSPSC Code:
12352107
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3563461
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grade

Molecular Biology

Quality Level

product line

BioUltra

form

liquid

storage condition

(Tightly closed.
Do not store near acidsTightly closed.
Do not store near acids)

concentration

~6 M in H2O

technique(s)

RNA extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

color

colorless

refractive index

n20/D 1.474-1.483

pH

4.5-7.0 (25 °C)

solubility

water: soluble

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤500 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

absorption

cut-off at 280 nm

suitability

suitable for molecular biology

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

General description

胍硫氰酸酯是一种离液剂,可用作强蛋白质变性剂。它被广泛应用于 RNA 分离方案。胍硫氰酸酯能不可逆地灭活 RNase 和 DNase 并破坏细胞膜。它通过扰乱蛋白质的二级结构来溶解蛋白质。胍硫氰酸酯还能将核蛋白从核酸中分离出来,并有助于蛋白酶对蛋白质的消化。它提高了蛋白酶 K 的活性,使其成为首选蛋白酶。

Application

胍硫氰酸酯溶液已用于提取:
  • 人类免疫缺陷病毒(HIV)感染和对照患者的血浆 RNA
  • 从收集的组织(芽、花、果实和种子)中提取总 RNA
  • 在逆转录聚合酶链反应(RT-PCR)分析中,从畸胎瘤细胞系(Tera-1)提取 RNA

Disclaimer

稍微加热至40-50℃,可能结晶的产物会再次溶解。


pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

存储类别

8A - Combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号GTIN
D120000004061833792865
46190-25MG04061832344683
50983-50ML04061832489957
50983-250ML04061832489940