Merck
CN

50983

Sigma-Aldrich

异硫氰酸胍水溶液 溶液

BioUltra, for molecular biology, ~6 M in H2O

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别名:
异硫氰酸胍, 硫氰酸亚脲, 硫氰酸胍
线性分子式:
NH2C(=NH)NH2 · HSCN
CAS号:
分子量:
118.16
Beilstein:
3563461
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

等级

for molecular biology

质量水平

产品线

BioUltra

浓度

~6 M in H2O

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

折射率

n20/D 1.474-1.483

pH值(酸碱度)

4.5-7.0 (25 °C)

痕量阴离子

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

吸光度

cut-off at 280 nm

SMILES字符串

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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一般描述

胍硫氰酸酯是一种离液剂,可用作强蛋白质变性剂。它被广泛应用于 RNA 分离方案。胍硫氰酸酯能不可逆地灭活 RNase 和 DNase 并破坏细胞膜。它通过扰乱蛋白质的二级结构来溶解蛋白质。胍硫氰酸酯还能将核蛋白从核酸中分离出来,并有助于蛋白酶对蛋白质的消化。它提高了蛋白酶 K 的活性,使其成为首选蛋白酶。

应用

胍硫氰酸酯溶液已用于提取:
  • 人类免疫缺陷病毒(HIV)感染和对照患者的血浆 RNA
  • 从收集的组织(芽、花、果实和种子)中提取总 RNA
  • 在逆转录聚合酶链反应(RT-PCR)分析中,从畸胎瘤细胞系(Tera-1)提取 RNA

注意

稍微加热至40-50℃,可能结晶的产物会再次溶解。

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

补充剂危害

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Viruses, 7(3), 939-968 (2015-03-10)
Human endogenous retrovirus (HERV-K (HML-2)) proviruses are among the few endogenous retroviral elements in the human genome that retain coding sequence. HML-2 expression has been widely associated with human disease states, including different types of cancers as well as with
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BMC genomics, 17(1), 771-771 (2016-10-08)
The parasitic flowering plant dwarf mistletoe (Arceuthobium spp., Viscaceae) is one of the most destructive forest pests, posing a major threat to numerous conifer species worldwide. Arceuthobium sichuanense (spruce dwarf mistletoe, SDM) infects Qinghai spruce (Picea crassifolia) and causes severe
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Recent studies suggest that human endogenous retrovirus group K (HERV-K) provirus expression plays a role in the pathogenesis of HIV-1 infection. In particular, RNA from the HML-2 subgroup of HERV-K proviruses has been reported to be highly expressed at the
Jeanine Griffin
Methods in molecular biology (Clifton, N.J.), 927, 379-384 (2012-09-21)
High quality DNA extractions developed for mammalian somatic cells are ineffective for sperm, due mainly to the high degree of nuclear compaction in sperm. The highly specialized nuclear proteins in sperm create a chromatin structure that is at least six
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Tendon healing is impaired in individuals diagnosed with diabetes mellitus (DM). According to research, there is considerable improvement in the healing of surgically tenotomized Achilles tendons following low-level laser therapy (LLLT) in non-diabetic, healthy animals. This study uses light microscopic

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