biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
concentration
~1 mg/mL
fluorescence
λex 638 nm; λem 655 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)
Application
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Other Notes
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
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