52544
Adenosine deaminase Streptococcus thermophilus, recombinant
recombinant, expressed in E. coli, ≥100 units/mL
别名:
ADA
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recombinant
expressed in E. coli
form
liquid
specific activity
≥100 units/mL
mol wt
38 kDa
storage temp.
−20°C
General description
Adenosine deaminase is a single polypeptide chain having eight parallel strands of α⁄β barrels. The molecular weight is found to be approximately 38,000 Da. The deep pocket at the COOH-terminal of the β barrel, which contains zinc as the co-factor, serves as the active site. It is ubiquitously found in wide variety of microorganisms, plants, and invertebrates.
Application
Adenosine deaminase was used to study its inhibitory effect on cerebrocortical vasodilatation (brain activation) and hypoxia. It was also used for the comparison of the inhibition by two adenosine analogs, N6-phenylisopropyladenosine and 5′-N-ethylcarboxamideadenosine of glycerol production by adipocytes
Biochem/physiol Actions
Adenosine deaminase is an active enzyme in purine metabolism, which mainly catalyzes the irreversible hydrolytic deamination of adenosine and deoxyadenosine to inosine and deoxyinosine, respectively. It is adequately present in mammalian tissue, and its deficiency leads to combined immunodeficiency disease. It is also present in all mammalian cells that play a key role in differentiation and maturation of lymphoid system.
Other Notes
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25°C.
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
E Dóra et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 4(3), 447-457 (1984-09-01)
The purpose of this study was to investigate the possible importance of adenosine in cerebrocortical vasodilatation accompanying brain activation (epileptic seizures and direct electrical stimulation) and hypoxia (arterial hypoxia and cyanide poisoning of the brain cortex). In chloralose-anesthetized cats a
C Londos et al.
Proceedings of the National Academy of Sciences of the United States of America, 77(5), 2551-2554 (1980-05-01)
Cell surface adenosine receptors mediate either stimulation or inhibition of adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1], and the receptors that mediate these different responses can be discriminated with selected adenosine analogs. 5'-N-Ethylcarboxamide-adenosine is a more potent agonist at
G Cristalli et al.
Medicinal research reviews, 21(2), 105-128 (2001-02-27)
Adenosine deaminase (ADA) is an enzyme of the purine metabolism which catalyzes the irreversible deamination of adenosine and deoxyadenosine to inosine and deoxyinosine, respectively. This ubiquitous enzyme has been found in a wide variety of microorganisms, plants, and invertebrates. In