Abberior STAR 600 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 600 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be effectively excited with excitation light between 550 - 610 nm. Abberior STAR 600 can substitute dyes like Atto^™ 590, Atto^™ 594 or Alexa Fluor^™ 594. For STED microscopy, Abberior STAR 600 can be most efficiently used with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 600 performs exceptionally well in combination with Abberior STAR 635P, STAR 635 or STAR RED. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 604 (PBS pH 7.4), 604 (H₂O), 601 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: : 95 000 (PBS pH 7.4), 95 000 (H₂O), 100 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,25 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 623 (PBS pH 7.4), 623 (H₂O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)

Designed and tested for fluorescent super-resolution microscopy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

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abberior is a registered trademark of Abberior GmbH