54287
Anti-Rabbit IgG−Abberior® CAGE 590 antibody produced in goat
for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
别名:
Abberior® CAGE 590-Anti-Rabbit IgG antibody produced in goat
产品名称
Anti-Rabbit IgG−Abberior® CAGE 590 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
biological source
goat
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
concentration
~1 mg/mL
fluorescence
λex 595 nm; λem 620 nm in PBS, pH 7.4 (photoactivated)
storage temp.
−20°C
Quality Level
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Application
Designed and tested for fluorescent super-resolution microscopy
General description
Abberior CAGE 590 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent orange-red dye.
It can most effectively be excited from 560 to 600 nm. Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE 590 can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 595 (photoactivated, PBS pH 7.4), 351 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 75 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 615 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, t [ns]: 4,0 (photoactivated, PBS pH 7.4)
It can most effectively be excited from 560 to 600 nm. Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE 590 can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 595 (photoactivated, PBS pH 7.4), 351 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 75 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 615 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, t [ns]: 4,0 (photoactivated, PBS pH 7.4)
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
此项目有
Rhodamines NN: a novel class of caged fluorescent dyes.
Vladimir N Belov et al.
Angewandte Chemie (International ed. in English), 49(20), 3520-3523 (2010-04-15)
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