57672
N-碘乙酰-N′-(5-磺基-1-萘)乙二胺
suitable for fluorescence, ≥95% (HPLC)
别名:
1,5-I-AEDANS, 5-[2-(碘乙酰氨基)乙氨基]萘-1-磺酸, N-碘乙酰基-N′-(5-磺基-1-萘基)乙二胺, Hudson-Weber 试剂
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关于此项目
线性分子式:
ICH2CONHCH2CH2NHC10H6SO3H
化学文摘社编号:
分子量:
434.25
UNSPSC Code:
12352200
PubChem Substance ID:
eCl@ss:
32035301
EC Number:
253-277-7
Beilstein/REAXYS Number:
3004454
MDL number:
InChI key
ZMERMCRYYFRELX-UHFFFAOYSA-N
InChI
1S/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21)
SMILES string
OS(=O)(=O)c1cccc2c(NCCNC(=O)CI)cccc12
assay
≥95% (HPLC)
fluorescence
λex 336 nm; λem 490 nm (pH 8.0), λex 337 nm; λem 498 nm (after derivatization with 2-mercaptoethanol)
suitability
suitable for fluorescence
storage temp.
2-8°C
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Application
N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid (1,5-I-AEDANS) is a fluorescence probe used to label protein sulfhydryls to study conformational changes, fluorescence anisotropy and protein:protein interactions.
Other Notes
Fluorescent sulfhydryl reagent
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Thorsten Beitlich et al.
PloS one, 8(10), e78384-e78384 (2013-11-10)
The globular 25 kDa protein cytosine monophosphate kinase (CMPK, EC ID: 2.7.4.14) from E. coli belongs to the family of nucleoside monophosphate (NMP) kinases (NMPK). Many proteins of this family share medium to high sequence and high structure similarity including
Taichi Yamamoto et al.
Journal of lipid research, 47(5), 1091-1096 (2006-02-10)
The low density lipoprotein receptor (LDLR) is the prototype of a family of cell surface receptors involved in a wide range of biological processes. A soluble low density lipoprotein receptor (sLDLR) and a tryptophan (Trp)-deficient variant human apolipoprotein E3 (apoE3)
Joseph J Kehoe et al.
Journal of agricultural and food chemistry, 55(17), 7107-7113 (2007-07-26)
This paper takes a new approach to determining which sulfhydryl groups are exposed during the heat denaturation of bovine beta-lactoglobulin A. The sulfhydryl groups exposed after heating were blocked with 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS). The results show that IAEDANS is a
Zofia Ostrowska-Podhorodecka et al.
Archives of biochemistry and biophysics, 682, 108280-108280 (2020-01-31)
Tropomyosin and cofilin are involved in the regulation of actin filament dynamic polymerization and depolymerization. Binding of cofilin changes actin filaments structure, leading to their severing and depolymerization. Non-muscle tropomyosin isoforms were shown before to differentially regulate the activity of
Anjali Jha et al.
Journal of molecular biology, 393(3), 735-752 (2009-09-01)
The aggregation of proteins often results in highly ordered fibrillar structures. While significant insights have been obtained on structural aspects of amyloid fibrils, little is known about the structures of protofibrils, which are presumed to be the precursors of fibrils.
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