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Merck
CN

59304

2-丙醇

greener alternative

≥99.5% (GC), for molecular biology, BioUltra

别名:

IPA, 仲丙醇, 异丙醇

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关于此项目

线性分子式:
(CH3)2CHOH
化学文摘社编号:
分子量:
60.10
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352104
EC Number:
200-661-7
MDL number:
Beilstein/REAXYS Number:
635639
Grade:
Molecular Biology
Assay:
≥99.5% (GC)
Technique(s):
DNA extraction: suitable
RNA extraction: suitable
Bp:
82 °C (lit.)
Vapor pressure:
33 mmHg ( 20 °C)
44 mmHg ( 25 °C)
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产品名称

2-丙醇, BioUltra, Molecular Biology, ≥99.5% (GC)

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

SMILES string

CC(C)O

grade

Molecular Biology

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

Quality Level

greener alternative product characteristics

Safer Solvents and Auxiliaries
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

evapn. residue

≤0.001%

color

colorless

refractive index

n20/D 1.377 (lit.)
n20/D 1.378

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤0.5 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

suitability

suitable for molecular biology

greener alternative category

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Application

2-丙醇被用于
  • 沉淀从苍蝇中提取的基因组DNA
  • 沉淀从移植原发肿瘤和器官中提取的基因组DNA
  • 免疫沉淀5-溴-2-脱氧尿苷(BrdU)

Biochem/physiol Actions

异丙醇(2-丙醇)在分子生物学中有广泛应用,用于从细胞和组织中提取沉淀后的DNA时。BioUltra IPA 适用于 DNA 沉淀。

Features and Benefits

  • 适用于生物化学和分子生物学的研究
  • BioUltra 级,经过严格的检测,符合严格的纯度标准
  • 不含 DNase, NICKase、RNase 和蛋白酶
  • 经过测试,确认低水平的重金属污染,确保各种应用的适用性

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This solvent is safe to use and thus aligns with "Safer Solvents and Auxiliaries". Click here for more information.

General description

2-丙醇是一种透明、无色的极性有机溶剂,常用于化学和生化实验室。它可溶解一系列的化物品并快速蒸发。异丙醇在分子生物学中常用于通过沉淀提取DNA和RNA。乙醇需要的体积是含核酸样品的2.5倍,而异丙醇可以等量加入。即使没有冷却,沉淀也会发生,使其有利于减少样品的总体体积。此外,它还应用于定量羟脯氨酸水平、通过MTT检测评估细胞活力,并有助于微阵列杂交过程。

Other Notes

如需了解生化试剂系列的更多信息,请填写此表

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Central nervous system

存储类别

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Spermine precipitation of DNA.
D.M. Wallace
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Better antibiotics capable of killing multi-drug-resistant Mycobacterium tuberculosis are urgently needed. Despite extensive drug discovery efforts, only a few promising candidates are on the horizon and alternative screening protocols are required. Here, by testing a panel of FDA-approved drugs in
H Dolatshad et al.
Leukemia, 29(5), 1092-1103 (2014-11-28)
The splicing factor SF3B1 is the most commonly mutated gene in the myelodysplastic syndrome (MDS), particularly in patients with refractory anemia with ring sideroblasts (RARS). We investigated the functional effects of SF3B1 disruption in myeloid cell lines: SF3B1 knockdown resulted
Thomas W Winkler et al.
PLoS genetics, 11(10), e1005378-e1005378 (2015-10-02)
Genome-wide association studies (GWAS) have identified more than 100 genetic variants contributing to BMI, a measure of body size, or waist-to-hip ratio (adjusted for BMI, WHRadjBMI), a measure of body shape. Body size and shape change as people grow older
Wei-Yu Lin et al.
Human molecular genetics, 24(1), 285-298 (2014-08-30)
Previous studies have suggested that polymorphisms in CASP8 on chromosome 2 are associated with breast cancer risk. To clarify the role of CASP8 in breast cancer susceptibility, we carried out dense genotyping of this region in the Breast Cancer Association

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