59767
氯酚红-β-D-吡喃半乳糖苷
β-galactosidase, ≥90% (HPLC), powder or crystals
别名:
CPRG, 氯酚红-β-D-半乳糖苷
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关于此项目
经验公式（希尔记法）:
C25H22Cl2O10S
化学文摘社编号:
分子量:
585.41
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
产品名称
氯酚红-β-D-吡喃半乳糖苷, ≥90% (HPLC)
SMILES string
OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
InChI key
YOUWVNCQJOMMEU-AETRGLENSA-N
assay
≥90% (HPLC)
form
powder or crystals
solubility
water: 20 mg/mL, clear, orange to very deep red
storage temp.
−20°C
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Application
氯酚红-β-D-吡喃半乳糖苷已被用作 β-半乳糖苷(β-ga）的底物：
- 用于靶RNA检测的基于纸的物细胞蛋白质表达体系
- 采用lacZ输出基因进行多个临床相关突变的比色检测
- 用于细菌定量的比色测定
General description
氯酚红-β-D-吡喃半乳糖苷(CPRG)是一种长波长黄色染料，常用于比色检测。它可作为 β-半乳糖苷酶 (β-gal) 的底物，半乳糖苷酶可水解CPRG 形成氯酚红（紫色）。该产品通过测量其在 570 nm 处的吸光度进行定量检测。CPRG 用于鉴定粪便大肠菌群。它的灵敏度比邻-硝基苯基-β-半乳糖苷（ONPG）等显色剂高10倍左右。CPRG还具有很高的快速转化率。
Other Notes
测量转染细胞中β-半乳糖苷酶的表达水平。lacZ基因是一种在转染反应中常用的报告基因，因为它的产物β-半乳糖苷酶是一种非常稳定的酶。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
涉药品监管产品
此项目有
Generation of antigen-specific, lacZ-inducible T-cell hybrids.
S Malarkannan et al.
Methods in molecular biology (Clifton, N.J.), 156, 265-272 (2000-11-09)
C Vega et al.
Parasitology research, 95(4), 296-298 (2005-02-01)
We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic beta-galactosidase released into the culture on membrane
V C Wutor et al.
Chemosphere, 68(4), 622-627 (2007-04-17)
The presence of coliforms in polluted water was determined enzymatically (in situ) by directly monitoring the activity of beta-d-galactosidase (B-GAL) through the hydrolysis of the yellow chromogenic subtrate, chlorophenol red beta-d-galactopyranoside (CPRG), which produced a red chlorophenol red (CPR) product.
D C Eustice et al.
BioTechniques, 11(6), 739-740 (1991-12-01)
A sensitive method has been developed for the detection of E. coli beta-galactosidase in transfected HeLa cells. The chromogenic substrate, CPRG (chlorophenol red-beta-D-galactopyranoside), was compared with ONPG (o-nitrophenyl-beta-D-galactopyranoside) by kinetic analysis with purified beta-galactosidase. The Km for CPRG was 1.35
J H Felgner et al.
The Journal of biological chemistry, 269(4), 2550-2561 (1994-01-28)
The application of cationic liposome reagents has advanced DNA and mRNA transfection research in vitro, and data are accumulating which show their utility for in vivo gene transfer. However, chemical structure-activity data leading to a better mechanistic understanding of their
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