61305
青霉素 来源于蜡样芽胞杆菌
lyophilized, powder, white, ~13 U/mg
别名:
β-内酰胺酶 I 来源于蜡样芽胞杆菌, β-内酰胺酶 II, 头孢菌素, 青霉素酰氨基-β-内酰胺水解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
~13 U/mg
Biological source:
Bacillus sp. (Bacillus cereus)
biological source
Bacillus sp. (Bacillus cereus)
form
powder
quality
lyophilized
specific activity
~13 U/mg
mol wt
Mr ~30000
technique(s)
activity assay: suitable
color
white
suitability
suitable for component for culture media
application(s)
diagnostic assay manufacturing
foreign activity
β-lactamase II ≤2%
storage temp.
−20°C
Quality Level
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General description
蜡样芽孢杆菌青霉素酶既是细胞外酶又是细胞结合酶。它具有单条多肽链，分子量为31 kDa。它在蛋白质序列中缺乏半胱氨酸。
Application
蜡样芽孢杆菌产青霉素酶，可用于制备安培检测青霉素水解的生物传感器
用于生产青霉素。
Biochem/physiol Actions
青霉素酶特异性催化青霉素的 β-内酰胺环的水解。
Packaging
无底玻璃瓶。内含物装在插入的融合锥内。
Other Notes
一个单元将在 pH7.0，25℃ 下，每分钟水解 1.0 μmole 指定的底物。以苄青霉素单位出售。该国际单位（使用苄青霉素作为底物）约等于 600 Levy 或 75 Pollock 单位。
用于破坏体液或培养基中的β-内酰胺类抗生素。用于青霉素的测定；参与异青霉素N合成酶的酶偶联测定法；雌二醇的ELISA；青霉素酶光极
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
W. Hobel et al.
Biosensors And Bioelectronics, 7, 549-549 (1992)
J E Baldwin et al.
Analytical biochemistry, 145(1), 183-187 (1985-02-15)
The development of a coupled enzyme assay for the determination of isopenicillin N synthetase activity in purified extracts from Cephalosporium acremonium was described. Isopenicillin N formed from its precursor, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV), by the synthetase was hydrolyzed by beta-lactamase I to
Penicillinase-based amperometric biosensor for penicillin G
Gonccalves LM, et al.
Electrochemical Communications, 38, 131-133 (2014)
Flow injection determination of penicillins using immobilized penicillinase in a single bead string reactor.
R Gnanasekaran et al.
Analytical chemistry, 57(6), 1005-1009 (1985-05-01)
T. Scheper et al.
Analytica Chimica Acta, 163, 111-111 (1984)
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