外形
aqueous solution with 4.5 mM triolein; 1 M NaCl; 13% (w/v) Triton™ X-100
其他说明
Assay of microbial lipases with emulsified trioleoyl glycerol; the fatty acids released are titrated with a pH stat
法律信息
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
A new assay of microbial lipases with emulsified trioleoyl glycerol.
N Peled et al.
Analytical biochemistry, 112(2), 219-222 (1981-04-01)
H Schmidinger et al.
Amino acids, 30(4), 333-350 (2006-06-15)
In the postgenomic era new technologies are emerging for global analysis of protein function. The introduction of active site-directed chemical probes for enzymatic activity profiling in complex mixtures, known as activity-based proteomics has greatly accelerated functional annotation of proteins. Here
Hannes Schmidinger et al.
Chembiochem : a European journal of chemical biology, 7(3), 527-534 (2006-02-14)
Protein and small-molecule microarrays are useful tools for high-throughput analysis of DNA-protein, protein-protein, and protein-small molecule interactions. Here we report on novel microarrays for activity screening of lipases and esterases based on phosphonic acid ester inhibitors. These compounds are activity
Maximilian Schicher et al.
Methods in molecular biology (Clifton, N.J.), 579, 497-511 (2009-09-19)
Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively
H Scholze et al.
Analytical biochemistry, 276(1), 72-80 (1999-12-10)
We report on the determination of active enzyme components in pure and crude lipases, using fluorescent inhibitors for covalent modification and visualization of the enzymatically active proteins. Lipase-specific compounds are triacylglycerol analogs, namely 1,2(2, 3)-di-O-alkylglyceroalkylphosphonic acid-p-nitrophenyl esters, containing a fluorescent
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