64958
2-甲氧基-2,4-二苯基-3(2H)-呋喃酮
suitable for fluorescence, ≥98.0% (HPLC)
别名:
2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF
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关于此项目
经验公式（希尔记法）:
C17H14O3
化学文摘社编号:
分子量:
266.29
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1288529
产品名称
2-甲氧基-2,4-二苯基-3(2H)-呋喃酮, suitable for fluorescence, ≥98.0% (HPLC)
InChI
1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3
SMILES string
COC1(OC=C(C1=O)c2ccccc2)c3ccccc3
InChI key
BLWINLJDTOJSRU-UHFFFAOYSA-N
assay
≥98.0% (HPLC)
form
solid
fluorescence
λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
Application
2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.
Other Notes
Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
新产品
此项目有
Pablo Castro-Hartmann et al.
Electrophoresis, 25(15), 2501-2505 (2004-08-10)
The high-energy intermediates generated in the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 can excite electronically different fluorophores with a high quantum yield in organic solvents. We have previously applied this peroxyoxalate chemiluminescent reaction to the detection of proteins labeled with
H. Nakamura et al.
Analytical Chemistry, 56, 919-919 (1984)
S. Stein
Pept. Neurobiol., 9-9 (1977)
V Toome et al.
Biochemical and biophysical research communications, 114(2), 433-439 (1983-07-29)
2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) reacts readily with the free amino group of a dipeptide to form a pyrrolinone-type chromophore with absorption maxima at 275-285 and 370-390 nm. A simple test tube procedure is described which allows in situ correlation of the absolute
Karen Martin et al.
Proteomics, 3(7), 1215-1227 (2003-07-23)
Three-color fluorescence detection methods are described based upon covalently coupling the dye 2-methoxy-2,4-diphenyl-2(2H)-furanone (MDPF) to proteins immobilized on poly(vinylidene difluoride) (PVDF) membranes, followed by detection of target proteins using alkaline-phosphatase-conjugated reporter molecules in combination with the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate
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