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Merck
CN

64958

Sigma-Aldrich

2-甲氧基-2,4-二苯基-3(2H)-呋喃酮

suitable for fluorescence, ≥98.0% (HPLC)

别名:

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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关于此项目

经验公式(希尔记法):
C17H14O3
化学文摘社编号:
分子量:
266.29
Beilstein:
1288529
MDL编号:
UNSPSC代码:
12352108
PubChem化学物质编号:
NACRES:
NA.32
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方案

≥98.0% (HPLC)

表单

solid

荧光

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES字符串

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

应用

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

其他说明

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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H. Nakamura et al.
Analytical Chemistry, 56, 919-919 (1984)
V Toome et al.
Biochemical and biophysical research communications, 114(2), 433-439 (1983-07-29)
2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) reacts readily with the free amino group of a dipeptide to form a pyrrolinone-type chromophore with absorption maxima at 275-285 and 370-390 nm. A simple test tube procedure is described which allows in situ correlation of the absolute
V E Urwin et al.
Analytical biochemistry, 195(1), 30-37 (1991-05-15)
A multiple mini two-dimensional electrophoretic method which results in three two-dimensional protein spot patterns being positioned side by side in an individual gel has been developed. Preparation time has been minimized by employing disposable capillary tubes for the isoelectric focusing
S. Stein
Pept. Neurobiol., 9-9 (1977)
R L O'Grady et al.
Analytical biochemistry, 140(2), 490-494 (1984-08-01)
This report describes the use of the compound 2-methoxy-2,4-diphenyl-3(2H)-furanone to label collagen as a substrate for the detection of mammalian collagenase in a fluorescent assay which is suitable for screening large numbers of samples. The compound 2-methoxy-2,4-diphenyl-3(2H)-furanone presents distinct advantages

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