Merck
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文件

69585

Sigma-Aldrich

4-甲基伞形酮基 N-乙酰基-β- D -氨基葡萄糖

suitable for fluorescence, ≥99.0% (TLC)

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别名:
4-甲基伞形酮-2-乙酰胺基-2-脱氧-β- D -吡喃葡萄糖苷
经验公式（希尔记法）:
C18H21NO8
CAS号:
37067-30-4
分子量:
379.36
Beilstein:
1693397
EC 号:
253-333-0
MDL编号:
MFCD00150977
PubChem化学物质编号:
57652199
NACRES:
NA.32

质量水平

100

检测方案

≥99.0% (TLC)

形式

powder

杂质

≤0.1% free 4-methylumbelliferone

溶解性

DMF: 20 mg/mL, clear, colorless

荧光

λex 317 nm (pH 10.0)
λex 365 nm; λem 445 nm in aqueous buffer pH 5.0 (after cleavage by β-N-acetylglucosaminidase)

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

[H]O[H].[H]O[H].CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2

InChI

1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-,16-,17-,18-;;/m1../s1

InChI key

PAVCYMSNMRWMAK-DMYIEBNJSA-N

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相关类别

应用

4-甲基伞形酮N-乙酰-β-D-氨基葡萄糖苷在酸性几丁质酶活性测定中用作荧光底物。它还用于测定水样滤液中β-N-乙酰己糖胺酶(NAGase)的总活性。

生化/生理作用

4-甲基伞形酮-N-乙酰-β-D-氨基葡萄糖苷(4-MUF-NAG)是一种用于氨基己糖苷酶的合成不带电荧光底物。它由几丁质单体（N-乙酰-β-D-氨基葡糖苷）和4-甲基伞形酮（7-氢氧基-4-甲基香豆素）(4-MUF)组成。4-MUF-NAG用于通过测定β-N-乙酰己糖胺酶(NAGase)活性来检测和估计真菌的生长。

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
β-N-乙酰氨基己糖苷酶的测定

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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J Charrow et al.
Clinical genetics, 27(1), 78-84 (1985-01-01)
A non-Jewish child with late onset GM2 gangliosidosis is described. Tissues from the patient had near normal hexosaminidase A (hex A) activity using 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-glcNAc) as substrate, and deficient activity when assayed with the 6-sulphate derivative of MU-glcNAc (MU-glcNAcS) or
Diogo Dias et al.
PloS one, 16(4), e0249541-e0249541 (2021-04-15)
Fish and other marine animals have a unique and intimate interaction with their surrounding environment. Diet type or trophic level play significant roles in modulating species digestive physiology. However, little is known about how the trophic niche influences digestive activity
B el Moudni et al.
Experimental parasitology, 83(2), 167-173 (1996-07-01)
N-Acetyl-beta-D-glucosaminidase activity was recovered in cell-free extracts of Trypanosoma cruzi epimastigotes. This enzyme was identified on the basis of its ability to hydrolyze the fluorogenic substrate 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide. This activity was purified to apparent homogeneity by anion exchange and molecular sieve
E C Landels et al.
Journal of medical genetics, 28(2), 101-109 (1991-02-01)
An assay for measuring hexosaminidase A in serum and leucocytes is described in which a centrifugal analyser is used for automation of the enzyme assays after manual heat inactivation. The assay was used in a screening programme to identify heterozygotes
N N Dewji et al.
The Biochemical journal, 234(1), 157-162 (1986-02-15)
beta-N-Acetylhexosaminidase I2 was purified from human liver by a combination of concanavalin A chromatography, DEAE-cellulose chromatography, gel filtration and affinity chromatography on 2-acetamido-N-(6-aminohexanoyl)-2-deoxy-beta-D-glucopyranosylamine coupled to CNBr-activated Sepharose 4B. Its specific activity was 130 mumol/min per mg of protein compared with
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