70540
萘酚黄 S
suitable for microscopy
别名:
2,4-二硝基-1-萘酚-7-磺酸 钠盐, 8-羟基-5,7-二硝基-2-萘磺酸 钠盐, 酸性黄 1, 黄胺酸 钠盐
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关于此项目
经验公式（希尔记法）:
C10H4N2Na2O8S
化学文摘社编号:
分子量:
358.19
颜色索引号:
10316
Beilstein:
3839220
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47
表单
powder
溶解性
methanol: water (1:1): 0.5 g/10 mL, clear
εmax
≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water
适用性
suitable for microscopy
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O
InChI
1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2
InChI key
CTIQLGJVGNGFEW-UHFFFAOYSA-L
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应用
Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.
生化/生理作用
Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.
分析说明
λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1 - STOT RE 2
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
P Skehan et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 21(5), 288-290 (1985-05-01)
A rapid method has been developed for measuring the cellular protein content of mono- and multilayered anchorage cultures. Fixed or air dried cultures are stained for 30 min with 0.2% Naphthol Yellow S (NYS) dissolved in 1% acetic acid. Unbound
S Gutschmidt et al.
Histochemistry, 72(3), 467-479 (1981-01-01)
In order to quantify the amount of protein in the small intestinal brush border region at different villus sites, cryostat sections of adult rat jejunum were stained with Naphthol Yellow S, Dinitrofluorobenzene and Coomassie Brilliant Blue and the dye deposits
J Gaub
The Histochemical journal, 13(5), 717-722 (1981-09-01)
Owing to the accumulation of nuclear non-histone protein (NHP) (a) in cells entering the cell cycle from the quiescent state and (b) in continuously cycling cells during G1 phase, a simultaneous determination of DNA and nuclear NHP is of high
F A Bignone et al.
Bollettino della Societa italiana di biologia sperimentale, 57(9), 950-955 (1981-05-15)
In the present work we studied the reversibility of the binding reaction of Naphthol Yellow S to proteins using a Testing Automatic System Leitz. We presently think that the NY-S staining procedure, can be used as a reliable quantitative method
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