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Merck
CN

70540

Sigma-Aldrich

萘酚黄 S

suitable for microscopy

别名:

2,4-二硝基-1-萘酚-7-磺酸 钠盐, 8-羟基-5,7-二硝基-2-萘磺酸 钠盐, 酸性黄 1, 黄胺酸 钠盐

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关于此项目

经验公式(希尔记法):
C10H4N2Na2O8S
化学文摘社编号:
分子量:
358.19
颜色索引号:
10316
Beilstein:
3839220
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47
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表单

powder

溶解性

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water

适用性

suitable for microscopy

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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应用

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

生化/生理作用

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

分析说明

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

象形图

Health hazardExclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1 - STOT RE 2

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
G Auer et al.
Analytical and quantitative cytology, 5(1), 1-4 (1983-03-01)
The ratio of protein to amount of DNA in the nucleus was cytophotometrically determined by means of a combined Feulgen-naphthol yellow S staining procedure. Quiescent normal lymphocytes, bronchial epithelial cells and mammary epithelial cells all showed ratios that were significantly
Cytospectral quantification for cellular dehydrogenases.
M O Yamada et al.
Cellular and molecular biology, including cyto-enzymology, 26(4), 347-352 (1980-01-01)
M S Huang et al.
Chest, 88(6), 864-869 (1985-12-01)
In order to study differences in the tumor growth and frequency of metastases between younger and older patients with lung cancer, we investigated the nuclear DNA and nuclear protein according to age by means of cytophotometry after combined staining with
J P Mitchell et al.
Histochemistry, 73(2), 211-223 (1981-01-01)
A comparison has been made between dinitrofluorobenzene (DNFB) and Naphthol Yellow S (NYS) as protein stains in combination with the pararosaniline-SO2 Feulgen procedure. Chicken erythrocytes were used as test cells. Cytophotometric measurements were made using a Zeiss scanning stage cytophotometer

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