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经验公式(希尔记法):
C13H19Br2N3O2
化学文摘社编号:
分子量:
409.12
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4630658
InChI key
FCRPMMVPBVOKQS-UHFFFAOYSA-M
SMILES string
[Br-].CC1=C(CBr)N2N(C1=O)C(=O)C(C)=C2C[N+](C)(C)C
InChI
1S/C13H19BrN3O2.BrH/c1-8-10(6-14)15-11(7-17(3,4)5)9(2)13(19)16(15)12(8)18;/h6-7H2,1-5H3;1H/q+1;/p-1
assay
≥90.0% (HPLC)
form
powder
solubility
DMSO: 5 mg/mL
fluorescence
λex 380 nm; λem 475 nm in 0.1 M phosphate pH 7.5
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
在与低分子量硫醇发生共轭反应之前,单溴二胺基本上是不发荧光的。与普通的单溴二胺不同,它带有正电荷,因此可以通过电泳或阳离子交换色谱法分离缀合物
Other Notes
用于标记生物材料中硫醇的试剂
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
S X Yuan et al.
FEBS letters, 234(1), 218-222 (1988-07-04)
The single cysteinyl residue 26 of spinach calmodulin was labelled with the thiol-specific bimane fluorescence probe. Following application of stoichiometric quantities of Ca2+ or aluminum ions to the protein, temperature-dependent fluorescence changes (anisotropy, lifetime) could be monitored via the label.
Conformational changes in mitochondrial aspartate aminotransferase detected by a covalently attached fluorescent probe.
E Sandmeier et al.
Progress in clinical and biological research, 144B, 117-124 (1984-01-01)
T T Huang et al.
Biology of reproduction, 31(4), 797-809 (1984-11-01)
The distribution of thiols and disulfides in the guinea pig spermatozoon during maturation and capacitation was studied using both membrane-permeable (mBBr) and impermeable (qBBr) forms of bromobimane, a specific fluorescent probe for thiol groups. In conjunction with the disulfide (SS)-reducing
Fluorescent localization of thiols and disulfides in marsupial spermatozoa by bromobimane labelling.
K E Mate et al.
Molecular reproduction and development, 37(3), 318-325 (1994-03-01)
The acrosome of marsupial spermatozoa is a robust structure which, unlike its placental counterpart, resists disruption by detergent or freeze/thawing and does not undergo a calcium ionophore induced acrosome reaction. In this study specific fluorescent thiol labels, bromobimanes, were used
Claresta Joe-Wong et al.
Environmental science & technology, 46(18), 9854-9861 (2012-08-25)
Organic thiols are highly reactive ligands and play an important role in the speciation of several metals and organic pollutants in the environment. Although small thiols can be isolated and their concentrations can be estimated using chromatographic and derivatization techniques
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