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73737

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

别名:

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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关于此项目

线性分子式:
(C6 H4 N O6 P)( C4 H12 N O3)2
化学文摘社编号:
68189-42-4
分子量:
461.36
NACRES:
NA.32
PubChem Substance ID:
57652488
UNSPSC Code:
12352204
EC Number:
269-198-6
MDL number:
MFCD00037009
Beilstein/REAXYS Number:
1578142
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产品名称

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐, for the determination of phosphatase, ≥97.0% (enzymatic)

Quality Level

100

assay

≥97.0% (enzymatic)

form

powder or crystals

quality

for the determination of phosphatase

impurities

≤0.1% free nitrophenol, ≤6% water

solubility

H2O: 0.1 g/mL, clear

storage temp.

2-8°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Application

4-硝基苯磷酸盐 双（三羟甲基氨基甲烷）盐被用于测定细胞裂解后酸性和碱性磷酸酶的酶活性。

Biochem/physiol Actions

4-硝基苯磷酸盐是一种非蛋白质和非特异性的显色底物， 是酶联免疫吸附试验（ELISA）中碱性磷酸酶（ALP）的首选。它广泛应用于测定蛋白酪氨酸磷酸酶（PTPs）。酶切后，底物产生黄色产物，可以采用分光光度计测定。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN1705
BCCD6826
BCCC2771
BCBW6778
BCBQ1676V

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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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Ulrike Lorenz
Current protocols in immunology, Chapter 11, Unit 11-Unit 11 (2011-04-05)
Tyrosine phosphorylation and dephosphorylation of proteins play a critical role in many processes of the immune system, from early development to fully differentiated effector function. Since the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) determine
D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
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货号GTIN
73737-5G04061832883595
73737-25G04061832921013