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Merck
CN

73737

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

别名:

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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关于此项目

线性分子式:
(C6 H4 N O6 P)( C4 H12 N O3)2
化学文摘社编号:
68189-42-4
分子量:
461.36
NACRES:
NA.32
PubChem Substance ID:
57652488
UNSPSC Code:
12352204
EC Number:
269-198-6
MDL number:
MFCD00037009
Beilstein/REAXYS Number:
1578142

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产品名称

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐, for the determination of phosphatase, ≥97.0% (enzymatic)

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

assay

≥97.0% (enzymatic)

form

powder or crystals

quality

for the determination of phosphatase

impurities

≤0.1% free nitrophenol, ≤6% water

solubility

H2O: 0.1 g/mL, clear

storage temp.

2-8°C

Quality Level

100

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Application

4-硝基苯磷酸盐 双（三羟甲基氨基甲烷）盐被用于测定细胞裂解后酸性和碱性磷酸酶的酶活性。

Biochem/physiol Actions

4-硝基苯磷酸盐是一种非蛋白质和非特异性的显色底物， 是酶联免疫吸附试验（ELISA）中碱性磷酸酶（ALP）的首选。它广泛应用于测定蛋白酪氨酸磷酸酶（PTPs）。酶切后，底物产生黄色产物，可以采用分光光度计测定。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN1705
BCCD6826
BCCC2771
BCBW6778
BCBQ1676V

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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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