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73837

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside

Name: 5-Bromo-4-chloro-3-indolyl &#945;-D-galactopyranoside
Brand: SIGMA

≥98.0% (HPLC), powder

别名:

X-α-Glc, X-α-glucoside

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关于此项目

经验公式（希尔记法）:

C₁₄H₁₅BrClNO₆

化学文摘社编号:

108789-36-2

分子量:

408.63

UNSPSC Code:

12352204

PubChem Substance ID:

329765272

NACRES:

NA.32

MDL number:

MFCD00155606

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属性

产品名称

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside, ≥98.0% (HPLC)

Quality Level

100

assay

≥98.0% (HPLC)

form

powder

optical activity

[α]/D 105.0±5.0°, c = 1 in DMF/H₂O 1:1

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11-,12+,13-,14+/m1/s1

InChI key

OPIFSICVWOWJMJ-HRNXZZBMSA-N

说明

General description

5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside, X-Gluc, is a indigogenic substrate for β- glucuronidase enzyme (GUS). It aids screening of transgenic plants based on GUS activity. GUS deesterifies X-Gluc into indoxyl derivative which upon oxidative polymerization results in the generation of blue indigotin dye.

Application

suitable as substrate for α-glucosidase; substrate used in the Druggan-Forsythe-Iversen medium (DFI agar) for the selective detection of Enterobacter sakazakii in infant milk

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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A Histochemical Method Using a Substrate of. BETA.-Glucuronidase for Detection of Genetically Modified Papaya

Wakui C, et al.

Shokuhin Eiseigaku Zasshi, 45(1), 19-24 (2004)

Molecular cloning and characterization of a Candida tsukubaensis alpha-glucosidase gene in the yeast Saccharomyces cerevisiae.

B T Kinsella et al.

Current genetics, 20(1-2), 45-52 (1991-07-01)

The molecular cloning of an alpha-glucosidase gene isolated from a Candida tsukubaensis (CBS 6389) genomic library in Saccharomyces cervisiae is reported. The cloned gene is contained within a 6.2 kb Sau3A DNA fragment and directs the synthesis and secretion of

GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Jefferson RA, et al.

The Embo Journal, 6(13), 3901-3907 (1987)

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全球贸易项目编号

货号	GTIN
73837-25MG	04061832921037
73837-100MG	04061832921020