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Merck
CN

75403

蛋白质结晶冷冻试剂盒

别名:

Protein Cryo Crystallography Kit

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UNSPSC Code:
12171500
Storage temp.:
2-8°C

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storage temp.

2-8°C

Application

The Crystallography Cryo Kit for Proteins provides a rapid, empirical, and efficient screening method to determine the solubility and optimal starting conditions for the crystallization of biological macromolecules in the presence of a cryoprotectant (primarily glycerol). The screening range and probing ability of the Cryo Kit are comparable to those of the Basic Kit. A cryoprotectant is added to freeze crystals in an amorphous glass at -173°C.

Biochem/physiol Actions

Based on known or published data from various proteins, the Cryo Kit uses a sparse matrix method and minimal amount of protein sample to explore a broad range of buffers, pH, and precipitants. The Protein Cryo Kit is based on the "salting out" principal: increasing the concentration of a precipitating reagent and cryoprotectant depletes the macromolecules of bound water and leads to their precipitation or crystallization.

Features and Benefits

  • Comprehensive, efficient screening
  • Stringent purity standards
  • Prefabricated reagents, flow charts, and observation sheets
  • Low incidence of undesirable solubility effects

Other Notes

The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.

仅试剂盒组分

产品编号
说明
  • Ammonium sulfate 0.17 M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% solution

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • 71591Imidazole pH 6.5; 0.07 M - Sodium acetate 0.7 M - Glycerol 30% solution化学品安全说明书
  • Sodium citrate tribasic pH 5.6; 0.07 M – Ammonium phosphate monobasic 0.7 M – Glycerol 30% (v/v) solution化学品安全说明书
  • 72872Magnesium chloride 0.18 M – HEPES-Na pH 7.5; 0.09M – 2-Propanol 27% (w/v) – Glycerol 10% solution化学品安全说明书
  • 74613Ammonium acetate 0.16M – TRIS hydrochloride pH 8.5; 0.08M – 2-Propanol 24% (v/v) – Glycerol 20% (v/v) solution化学品安全说明书
  • Magnesium chloride 0.2M – HEPES Na pH 7.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution化学品安全说明书
  • HEPES Na pH 7.5; 0.065M – Potassium sodium tartrate 0.52M – Glycerol 35% (v/v) solution化学品安全说明书
  • Ammonium acetate 0.17M – Sodium acetate pH 4.6; 0.085M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution化学品安全说明书
  • Magnesium acetate 0.2M – Sodium cacodylate pH 6.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution化学品安全说明书
  • Sodium citrate tribasic 0.2M – TRIS hydrochloride pH 8.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution化学品安全说明书
  • 80074Ammonium sulfate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution化学品安全说明书
  • Lithium sulfate 0.17 M – TRIS hydrochloride pH 8.5; 0.085M – Polyethylen glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution化学品安全说明书
  • Magnesium acetate 0.16 M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% solution化学品安全说明书
  • 80384Sodium citrate tribasic 0.14M, HEPES Na pH 7.5; 0.07M, 2-Propanol 14% (v/v), Glycerol 30% solution化学品安全说明书
  • 80387Sodium acetate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution化学品安全说明书
  • Calcium chloride 0.19 M – HEPES-Na pH 7.5; 0.095M – Polyethylene glycol 400; 26.6% (w/v) – Glycerol 5% solution化学品安全说明书
  • HEPES-Na pH 7.5; 0.085M – Polyethylene glycol 400 1.7% (v/v) – Ammonium sulfate 1.7M – Glycerol 15% (v/v) solution化学品安全说明书
  • HEPES-Na pH 7.5; 0.075M – Sodium phosphate monobasic 0.6M – Potassium phosphate monobasic 0.6M – Glycerol 25% (v/v) solution化学品安全说明书
  • Lithium sulfate 0.4 M – Polyethylene glycol 8000 12% (w/v) – Glycerol 20% (v/v) solution化学品安全说明书
  • 81892Sodium formate 3.6 M – Glycerol 10% solution化学品安全说明书
  • Sodium acetate pH 4.6; 0.07M – 1.4 M Sodium formate – Glycerol 30% (v/v) solution化学品安全说明书
  • HEPES-Na pH 7.5; 0.085M – 2-Propanol 8.5% (v/v) – Polyethylene glycol 4000 17% (w/v) – Glycerol 15% (v/v) solution化学品安全说明书
  • 83196Sodium acetate pH 4.6; 0.08 M – Ammonium sulfate 1.6 M – Glycerol 20% (v/v) solution化学品安全说明书
  • Potassium phosphate monobasic 0.04M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% (v/v) solution化学品安全说明书
  • TRIS hydrochloride pH 8.5; 0.08 M – Ammonium phosphate monobasic 1.6 M – Glycerol 20% (v/v) solution化学品安全说明书
  • 89252Ammonium sulfate 1.5 M – Glycerol 25% solution化学品安全说明书
  • HEPES-Na pH 7.5; 0.09M – Sodium citrate tribasic 1.26M – Glycerol 10% solution化学品安全说明书
  • Polyethylene glycol 1500 24% (w/v) – Glycerol 20% (v/v) solution化学品安全说明书
  • 91638Magnesium formate 0.1M – Glycerol 50% (v/v) solution化学品安全说明书
  • Calcium chloride 0.02 M – Sodium acetate 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution化学品安全说明书
  • Sodium citrate tribasic 0.2M – HEPES-Na pH 7.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution化学品安全说明书
  • TRIS hydrochloride pH 8.5; 0.075 M – Ammonium sulfate 1.5 M – Glycerol 25% (v/v) solution化学品安全说明书
  • Ammonium phosphate monobasic 0.26 M – Glycerol 35% (v/v) solution化学品安全说明书
  • 96864Potassium sodium tartrate 0.26 M – Glycerol 35% (v/v) solution化学品安全说明书
  • Calcium chloride 0.14M – Sodium acetate pH 4.6; 0.07M – 2-Propanol 14% (v/v) – Glycerol 30% (v/v) solution化学品安全说明书
  • Sodium cacodylate pH 6.5; 0.07M – Sodium acetate 0.98M – Glycerol 30% (v/v) solution化学品安全说明书
  • Ammonium sulfate 0.16 M – Sodium acetate pH 4.6; 0.08M – Polyethylene glycol 4000 20% (w/v) – Glycerol 20% (v/v) solution化学品安全说明书
  • 80084HEPES Na pH 7.5; 0.075M – Lithium sulfate 1.125M – Glycerol 25% (v/v) solution化学品安全说明书
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Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

3 - Flammable liquids

法规信息

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分析证书(COA)

Lot/Batch Number
BCBB3511

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Garman, E.F. and Mitchell, E.P.
J. Appl. Cryst., 29, 584-584 (1996)

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