conjugate
Atto 594 conjugate
antibody product type
secondary antibodies
clone
polyclonal
form
liquid
contains
50% glycerol as stabilizer
species reactivity
rabbit
concentration
~1 mg/mL protein
fluorescence
λex 607 nm; λem 626 nm in PBS
suitability
corresponds for gel electrophoresis
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio ≥2
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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signalword
Warning
hcodes
pcodes
Hazard Classifications
Eye Irrit. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
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Thorsten G Müller et al.
eLife, 10 (2021-04-28)
HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization, and mechanism of uncoating are under debate. We adapted a strategy to visualize individual reverse-transcribed HIV-1 cDNA molecules and their association with viral and cellular proteins using fluorescence and
Mirko Cortese et al.
Cell host & microbe, 28(6), 853-866 (2020-11-28)
Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation-dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2-infected human lung epithelial cells.
Krzysztof Marycz et al.
International journal of nanomedicine, 16, 6049-6065 (2021-09-14)
Healing of osteoporotic defects is challenging and requires innovative approaches to elicit molecular mechanisms promoting osteoblasts-osteoclasts coupling and bone homeostasis. Cytocompatibility and biocompatibility of previously characterised nanocomposites, i.e Ca5(PO4)3OH/Fe3O4 (later called nHAp/IO) functionalised with microRNAs (nHAp/IO@miR-21/124) was tested. In vitro
Visar Ajeti et al.
Nature physics, 15, 696-705 (2020-01-04)
How cells with diverse morphologies and cytoskeletal architectures modulate their mechanical behaviors to drive robust collective motion within tissues is poorly understood. During wound repair within epithelial monolayers in vitro, cells coordinate the assembly of branched and bundled actin networks
Lena K Schroeder et al.
The Journal of cell biology, 218(1), 83-96 (2018-11-18)
The endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Superresolution microscopy recently revealed densely packed, rapidly moving ER tubules mistaken for sheets by conventional light microscopy, highlighting the importance of revisiting classical views of ER structure with
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Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。
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