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文件

79390

Sigma-Aldrich

磷酸酶(碱性) 来源于小牛肠粘膜

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

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别名:
Alkaline Phosphatase from calf intestinal mucosa
CAS号:
9001-78-9
EC 号:
3.1.3.1 (BRENDA, IUBMB)
EC 号:
232-631-4
MDL编号:
MFCD00131849
eCl@ss:
42010105
NACRES:
NA.54

生物来源

bovine (calf) intestinal mucosa

形式

solution (clear, colorless)

比活

~2500 U/mg protein (~10 mg/ml)

分子量

Mr ~140000

技术

enzyme immunoassay: suitable

杂质

0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine

pH值（酸碱度）

~7.6

储存温度

2-8°C

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相关类别

应用

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

生化/生理作用

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

单位定义

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

重悬

The solution can be used without dialysis (for enzyme immuno-assay).

其他说明

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
K.R. Wehrmeyer et al.
Journal of Liquid Chromatography, 6, 2141-2141 (1983)
C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
Opioid receptor agonists enhance the phosphorylation state of Fas-associated death domain (FADD) protein in the rat brain: functional interactions with casein kinase Ialpha, Galpha(i) proteins, and ERK1/2 signaling.
Garcia-Fuster, M.J., et al.
Neuropsychopharmacology, 55, 886-899 (2008)
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