磷酸酶(碱性) 来源于小牛肠粘膜

Name: 磷酸酶(碱性) 来源于小牛肠粘膜
Brand: SIGMA

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

别名:

Alkaline Phosphatase from calf intestinal mucosa

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About This Item

CAS Number:

9001-78-9

EC 号:

3.1.3.1 (BRENDA, IUBMB)

EC 号:

232-631-4

MDL编号:

MFCD00131849

UNSPSC代码:

12352204

eCl@ss:

42010105

NACRES:

NA.54

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生物来源

bovine (calf) intestinal mucosa

表单

solution (clear, colorless)

比活

~2500 U/mg protein (~10 mg/ml)

分子量

M_r ~140000

技术

enzyme immunoassay: suitable

杂质

0.1 mM ZnCl₂
1 mM MgCl₂
3 M NaCl
30 mM triethanolamine

pH值（酸碱度）

~7.6

储存温度

2-8°C

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应用

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

生化/生理作用

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

制备说明

The solution can be used without dialysis (for enzyme immuno-assay).

其他说明

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase