83330
丙酮酸激酶 来源于兔肌肉
crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)
别名:
ATP：丙酮酸盐 2-O-磷酸转移酶, PK
表单
suspension
质量
crystalline suspension in 3.8 M ammonium sulfate solution
比活
~350 U/mg protein (10-40 mg/ml)
分子量
Mr ~230000
pH值（酸碱度）
~6
异质活性
lactate dehydrogenase ≤0.01%
储存温度
2-8°C
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生化/生理作用
分子量：237 kDa，以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.
其他说明
1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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