83330
丙酮酸激酶 来源于兔肌肉
crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)
别名:
ATP：丙酮酸盐 2-O-磷酸转移酶, PK
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-616-2
NACRES:
NA.54
MDL number:
Specific activity:
~350 U/mg protein (10-40 mg/ml)
form
suspension
quality
crystalline suspension in 3.8 M ammonium sulfate solution
specific activity
~350 U/mg protein (10-40 mg/ml)
mol wt
Mr ~230000
pH
~6
foreign activity
lactate dehydrogenase ≤0.01%
storage temp.
2-8°C
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Biochem/physiol Actions
分子量：237 kDa，以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.
Other Notes
1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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