Clone 1-5c-4 cell line (HeLa deriative)

Plaque assays, cloning, genetic and virus studies: swine influenza viruses

Human cervix carcinoma

These cells were originally Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival ′D′ cells. Susceptible to swine influenza viruses, supports production of plaque formation by a variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Epithelial with occasional giant cells. Ethnicity: Black.

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using EDTA or 0.25% trypsin; 5% CO₂; 37°C.

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RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.