等级
Molecular Biology
质量水平
产品线
BioReagent
表单
solution
技术
Southern blotting: suitable
杂质
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
pH值（酸碱度）
7.0±0.2
密度
1.16 g/mL at 20 °C
储存温度
room temp
一般描述
SSC缓冲液20倍浓缩液是Southern和Northern印迹法的标准试剂。该浓缩配方可直接使用，或稀释后制成浓度低至0.5倍的SSC工作溶液。
应用
适用于：
- 制备用于杂交的核酸
- 制备用于Northern和Southern印迹的杂交缓冲液
- 制备用于Northern和Southern印迹的洗涤缓冲液
特点和优势
- 方便的即用型液体
- 在杂交中具有多种用途
- 可轻松稀释至所需浓度
制备说明
SSC缓冲液20倍浓缩液的溶剂为含有0.3 M柠檬酸钠的3M NaCl。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
Guanghua Yang et al.
BMC biology, 11, 86-86 (2013-07-23)
Human pluripotent stem cells (hPSCs) hold great promise for applications in regenerative medicine. However, the safety of cell therapy using differentiated hPSC derivatives must be improved through methods that will permit the transplantation of homogenous populations of a specific cell
Sterghios A Moschos et al.
BMC genomics, 8, 240-240 (2007-07-21)
At present, nothing is known of the role of miRNAs in the immune response in vivo despite the fact that inflammation is thought to underlie multiple acute and chronic diseases. In these circumstances, patients are commonly treated with corticosteroids such
商品
Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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