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Merck
CN

93359

Tris 乙磺酸

BioUltra, ≥99.5% (calc. based on dry substance, T)

别名:

2-[[三(羟甲基)甲基]氨基]乙磺酸, N-三(羟甲基)甲基-2-氨基乙烷磺酸, TES 游离酸

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关于此项目

经验公式(希尔记法):
C6H15NO6S
化学文摘社编号:
分子量:
229.25
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
230-906-3
Beilstein/REAXYS Number:
1957061
MDL number:
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InChI key

JOCBASBOOFNAJA-UHFFFAOYSA-N

InChI

1S/C6H15NO6S/c8-3-6(4-9,5-10)7-1-2-14(11,12)13/h7-10H,1-5H2,(H,11,12,13)

SMILES string

OCC(CO)(CO)NCCS(O)(=O)=O

product line

BioUltra

assay

≥99.5% (calc. based on dry substance, T)

form

powder

impurities

insoluble matter, passes filter test

ign. residue

≤0.1% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

3.5-5.0 (25 °C, 1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

mp

~225 °C (dec.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤200 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤100 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.055, λ: 280 nm Amax: 0.045

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Application

TES is a physiological Good′s buffer (pH 74.-7.9) used in biological research applications. It is often compared to other Good′s buffers for chemistry and optimal performance in a given system.

Other Notes

Hydrogen ion buffer for biological research; Suitable buffer for epidermal cell growth at pH 7.4-7.9 where additional buffering capacity is required

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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H. Hammar et al.
Epidermis Dis., Proc. Eur. Soc. Dermatol. Res. Symp. 1979, 243-243 (1981)
U Kragh-Hansen et al.
Clinical chemistry, 39(2), 202-208 (1993-02-01)
We examined the suitability of nine organic buffers for studying calcium binding to albumin by equilibrium dialysis. Results obtained with defatted human serum albumin showed that 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 2-([tris(hydroxymethyl)methyl] amino)ethanesulfonic acid were superior. Scatchard analysis of the experimental
G S Prins et al.
Fertility and sterility, 46(1), 147-149 (1986-07-01)
Several cryoprotective media currently in use for human sperm cryopreservation have been directly compared with each other by use of a static vapor freezing technique. The TEST-citrate-yolk buffers with glycerol resulted in the highest motility and longevity after thawing; thus
N Sugiyama et al.
Molecular reproduction and development, 58(1), 30-38 (2001-01-06)
The growth, differentiation, and death/survival of spermatogonia are precisely regulated for the proper production of spermatozoa. We have previously shown that Bcl-2 ectopically expressed in spermatogonia caused the inhibition of normal spermatogonial apoptosis and the subsequent failure of differentiation in
R H Martin et al.
Biology of reproduction, 47(2), 268-270 (1992-08-01)
Human sperm karyotypes can be prepared after fusion of human sperm with Golden hamster oocytes. Most laboratories use one of two methods of sperm capacitation: incubation of freshly-ejaculated sperm in Biggers, Whitten, and Whittingham (BWW) medium for 5-7 h at

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