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Merck
CN

93711

Atto 655

BioReagent, suitable for fluorescence, ≥85% (HPLC)

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关于此项目

UNSPSC Code:
12352116
PubChem Substance ID:
57653573
NACRES:
NA.32
MDL number:
MFCD03456136

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产品名称

Atto 655, BioReagent, suitable for fluorescence, ≥85% (HPLC)

SMILES string

[S](=O)(=O)([O-])CC1CC(N(c2c1cc3c(c2)OC4=CC5=[N+](CCCC5=CC4=N3)CC)CCCC(=O)O)(C)C

InChI

1S/C27H33N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-14,18H,4-10,15-16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

FOYVTVSSAMSORJ-UHFFFAOYSA-N

product line

BioReagent

assay

≥85% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
655 nm

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 652-658 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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Application

Atto 标签适用于灵敏度最高的应用。独特的优势组合使其成为各种标签应用的极佳工具。它们的一些特性使它们特别适用于单分子检测。Atto 标签基于刚性结构，并且不显示任何顺式-反式-异构化，这降低了信号的亮度，并导致环境依赖性，例如共轭光谱移位。
Atto 655 在水中摩尔消光为 110,000，在含 50% 乙醇的水溶液中 QY 为 30％。衰变时间是 1.9 ns。

Other Notes

新型红色吸收荧光染料，具有最佳的信噪比和长荧光寿命。可用作结合相互作用的生物物理探针。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCP0768
BCCN8603
BCCN5830
BCCN4662
BCCN3519

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Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
Bengang Xing et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 17(50), 14170-14177 (2011-11-16)
The molecular interactions of the glycopeptide antibiotic vancomycin (Van) with bacterial cell wall analogues N,N'-diacetyl-L-Lys-D-Ala-D-Ala (Ac(2) KdAdA) and N,N'-diacetyl-L-Lys-D-Ala-D-Lac (Ac(2) KdAdL) were investigated in neat water, phosphate buffer and HEPES buffer by using fluorescence correlation spectroscopy (FCS) and molecular dynamics
Katharina Stöhr et al.
Analytical chemistry, 77(22), 7195-7203 (2005-11-16)
Due to growing problems with new emerging pathogens, cost-effective and manageable methods for their accurate identification in routine diagnostics are urgently required. Of particular importance is the genus Mycobacterium with its more than 100 species. Identification of these species is
Zhixing Chen et al.
Journal of the American Chemical Society, 134(33), 13692-13699 (2012-08-10)
Chemical tags are now viable alternatives to fluorescent proteins for labeling proteins in living cells with organic fluorophores that have improved brightness and other specialized properties. Recently, we successfully rendered our TMP-tag covalent with a proximity-induced reaction between the protein
Fabian Heinemann et al.
Langmuir : the ACS journal of surfaces and colloids, 28(37), 13395-13404 (2012-08-16)
Fluorescence correlation spectroscopy (FCS) measurements are widely used for determination of diffusion coefficients of lipids and proteins in biological membranes. In recent years, several variants of FCS have been introduced. However, a comprehensive comparison of these methods on identical systems
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商品

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

荧光寿命测量

荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。

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