Merck
CN

94046

Sigma-Aldrich

磷酸氢二钠 溶液

BioUltra, 0.5 M in H2O

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别名:
磷酸氢二钠 溶液
线性分子式:
Na2HPO4
CAS号:
分子量:
141.96
MDL编号:
eCl@ss:
38070211
PubChem化学物质编号:
NACRES:
NA.25

产品线

BioUltra

形式

liquid

浓度

0.5 M in H2O

杂质

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH值(酸碱度)

8.8-9.4 (25 °C, 0.5 M in H2O)

溶解性

H2O: 0.5 M at 20 °C, clear, colorless

密度

1.064 g/mL at 20 °C

痕量阴离子

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

紫外吸收

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

应用

diagnostic assay manufacturing

SMILES字符串

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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应用

磷酸氢二钠溶液可用于制备多种磷酸盐基生物缓冲液,用于细胞和分子生物学应用。
磷酸氢二钠溶液已用于:
  • 在钙化培养基中添加Dulbecco′改良伊格尔培养基(DMEM),以诱导血管平滑肌细胞(VSMC)的钙化
  • 制备CaCl2和 MgCl2的原液,以合成磷酸钙(CPB)和磷酸镁(MPB)
  • 补充2xYT琼脂板以培养BL21 DE3星型细胞和BL21dLac Gold型细胞

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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Daria Shishkova et al.
International journal of molecular sciences, 21(22) (2020-11-26)
Calciprotein particles (CPPs), which increasingly arise in the circulation during the disorders of mineral homeostasis, represent a double-edged sword protecting the human organism from extraskeletal calcification but potentially causing endothelial dysfunction. Existing models, however, failed to demonstrate the detrimental action
Janelle M Spinazzola et al.
Human molecular genetics, 24(9), 2470-2481 (2015-01-22)
Loss of gamma-sarcoglycan (γ-SG) induces muscle degeneration and signaling defects in response to mechanical load, and its absence is common to both Duchenne and limb girdle muscular dystrophies. Growing evidence suggests that aberrant signaling contributes to the disease pathology; however
Megan A Rees et al.
Journal of proteome research, 14(1), 120-132 (2014-10-21)
Interactions between a host and a bacterial pathogen are mediated by cross-talk between molecules present on, or secreted by, pathogens and host binding-molecules. Identifying proteins involved at this interface would provide substantial insights into this interaction. Although numerous studies have
Natalie K Binder et al.
Molecular human reproduction, 21(5), 424-434 (2015-03-04)
The rate of obesity among men of reproductive age has tripled in the last three decades. Previously, we demonstrated that paternal obesity resulted in impaired preimplantation developmental kinetics, compromised post-compaction metabolism and decreased blastocyst cell number when embryos were generated
Laure Thibaudeau et al.
Disease models & mechanisms, 7(2), 299-309 (2014-04-10)
The skeleton is a preferred homing site for breast cancer metastasis. To date, treatment options for patients with bone metastases are mostly palliative and the disease is still incurable. Indeed, key mechanisms involved in breast cancer osteotropism are still only

实验方案

This procedure may be used for Hyaluronidase products.

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

在测定醇脱氢酶活性时,该测定使用β-烟酰胺腺嘌呤二核苷酸磷酸和在340nm处的连续分光光度法进行测定。

在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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