General description
亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
由六个亚基组成,总分子量:约 544620
脲酶(Ure)酶存在于胞质溶胶中。它在酸性 pH 下具有活性,在 28℃ 下表达。脲酶由镍组成,存在于古细菌、细菌、单细胞真核生物和植物中。
Application
来自洋刀豆的脲酶可用于各种样品的尿素测定,例如豆类。它可用于检测病原体以及重金属离子。
来自洋刀豆(Jack bean)的脲酶已用于纯化和结晶研究。
Biochem/physiol Actions
脲酶催化尿素水解成二氧化碳和氨。脲酶参与氮代谢和尿素降解。来自洋刀豆的脲酶每个亚基结合 2 个镍离子。
Other Notes
1U 对应于在 pH 8.0 和 25℃ 下每分钟水解 1 μmol 尿素(货号 51459)所需的酶量。
Application in (selective) hydrolysis/condensations;citation structure.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
低风险生物材料
此项目有
I.A. Natchev
Tetrahedron, 44, 1511-1511 (1988)
Isothermal titration calorimetry to characterize enzymatic reactions
Methods in Enzymology, 567, 215-236 (2016)
M J Sculley et al.
Biophysical chemistry, 19(1), 39-47 (1984-01-01)
The use of bifunctional reagents to form cross-links between subunits in protein oligomers and subsequent disruption of noncovalent interactions with SDS allows comment upon the number of subunits and the symmetry in the original assembly. In existing treatments the number
K Takishima et al.
European journal of biochemistry, 175(1), 151-165 (1988-07-15)
The amino acid sequence of jack bean urease has been determined. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease
R.L. Blakeley et al.
J. Mol. Catal., 23, 263-263 (1984)
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