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Merck
CN

94403

Hoechst 33258 溶液

1 mg/mL in H2O, ≥98.0% (HPLC)

别名:

双苯并咪唑H 33258溶液, 赫斯特荧光染料 33258 溶液

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关于此项目

经验公式（希尔记法）:
C25H24N6O · 3HCl
化学文摘社编号:
23491-45-4
分子量:
533.88
MDL number:
MFCD29074011
UNSPSC Code:
12171500
PubChem Substance ID:
329770334
NACRES:
NA.32

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产品名称

Hoechst 33258 溶液, 1 mg/mL in H2O, ≥98.0% (HPLC)

SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

assay

≥98.0% (HPLC)

form

liquid

Quality Level

100

mol wt

533.88 g/mol

concentration

1 mg/mL in H2O

color

colorless
very light green-yellow to dark green-yellow
very light yellow to dark yellow

pH

6.0-8.0 (25 °C)

solubility

DMF: soluble
H2O: soluble

density

1.00 g/cm3

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

storage temp.

2-8°C

Application

赫斯特 33258被用于荧光法估算样品中DNA浓度，这种方法比分光光度法更好，它可以测定纳克级DNA。并且，由于赫斯特 33258适用于识别细胞培养基中的支原体污染，由于其高荧光性。

General description

赫斯特 33258，也被称为双联苯亚胺，是一种蓝色荧光染料。它被归类为一种染色体染剂，可与DNA双螺旋的小沟结合，识别一组四对A-T碱基对。

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN9215
BCCN1642
BCCN0204
BCCM2644
BCCL1611

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A Fede et al.
Structure (London, England : 1993), 1(3), 177-186 (1993-11-15)
The chromosomal stain, Hoechst 33258, binds to the minor groove of the DNA double helix and specifically recognizes a run of four A-T base pairs. Extensive biochemical and biophysical studies have been aimed at understanding the binding of the dye
Cornelia Fritsch et al.
PLoS genetics, 15(7), e1008269-e1008269 (2019-07-13)
Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor
Natalia Busto et al.
The journal of physical chemistry. B, 119(13), 4575-4581 (2015-03-12)
The functionality of the bisbenzimide Hoechst 33258 in solution has been largely exploited in the quantification of DNA. Understanding of its behavior is essential to promote its widespread application and learning of biological processes. A detailed study of the dimerization
Michael R Green et al.
Cold Spring Harbor protocols, 2017(5) (2017-05-04)
Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
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