跳转至内容
Merck
CN

94403

Sigma-Aldrich

Hoechst 33258 溶液

1 mg/mL in H2O, ≥98.0% (HPLC)

别名:

双苯并咪唑H 33258溶液, 赫斯特荧光染料 33258 溶液

登录查看公司和协议定价

选择尺寸

关于此项目

经验公式（希尔记法）:
C25H24N6O · 3HCl
化学文摘社编号:
23491-45-4
分子量:
533.88
MDL编号:
MFCD29074011
UNSPSC代码:
12171500
PubChem化学物质编号:
329770334
NACRES:
NA.32

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

质量水平

100

方案

≥98.0% (HPLC)

表单

liquid

浓度

1 mg/mL in H2O

溶解性

DMF: soluble
H2O: soluble

荧光

λex 355 nm; λem 465 nm in TE buffer; DNA

储存温度

2-8°C

SMILES字符串

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

一般描述

赫斯特 33258，也被称为双联苯亚胺，是一种蓝色荧光染料。它被归类为一种染色体染剂，可与DNA双螺旋的小沟结合，识别一组四对A-T碱基对。

应用

赫斯特 33258被用于荧光法估算样品中DNA浓度，这种方法比分光光度法更好，它可以测定纳克级DNA。并且，由于赫斯特 33258适用于识别细胞培养基中的支原体污染，由于其高荧光性。

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN1642
BCCN0204
BCCM2644
BCCL1611
BCCK6782

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Natalia Busto et al.
The journal of physical chemistry. B, 119(13), 4575-4581 (2015-03-12)
The functionality of the bisbenzimide Hoechst 33258 in solution has been largely exploited in the quantification of DNA. Understanding of its behavior is essential to promote its widespread application and learning of biological processes. A detailed study of the dimerization
Cornelia Fritsch et al.
PLoS genetics, 15(7), e1008269-e1008269 (2019-07-13)
Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor
A Fede et al.
Structure (London, England : 1993), 1(3), 177-186 (1993-11-15)
The chromosomal stain, Hoechst 33258, binds to the minor groove of the DNA double helix and specifically recognizes a run of four A-T base pairs. Extensive biochemical and biophysical studies have been aimed at understanding the binding of the dye
Michael R Green et al.
Cold Spring Harbor protocols, 2017(5) (2017-05-04)
Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.
T R Chen
Experimental cell research, 104(2), 255-262 (1977-02-01)
查看所有相关文献

实验方案

Human Neural Stem Cells Differentiation with TrueGel3D®

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持