assay
≥80.0%
form
solid
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 640 nm; λem 655 nm±10 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)
Application
- Abberior® Star 635 maleimide has been used for single-molecule FRET (Förster resonance energy transfer) spectroscopy for protein dynamics in live cells.
- Abberior® STAR 635 goat anti-rabbit antibody has been used for STED (stimulated emission depletion) imaging of primary cultured striatal neurons obtained from rats.
- Abberior® STAR 635 anti-rabbit antibody has been used for STED imaging of non-sensory supporting cells obtained from cochlea of rats and mice.
- Abberior® STAR 635 conjugated with secondary antibody has been used for STED imaging of microtubules in cells.
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable