96667
酯酶 来源于枯草芽孢杆菌
recombinant, expressed in E. coli, ≥10 U/mg
别名:
Carboxylesterase
一般描述
酯酶属于水解酶超家族。该重组酯酶含有一个C末端组氨酸标签。
应用
来自枯草芽孢杆菌的酯酶可用于蛋白质工程研究以及对芳基脂族叔醇乙酸酯的动力学分辨进行研究。产品96667是经重组并大肠杆菌（≥10 U/mg）中表达的。
生化/生理作用
酯酶可参与酯的立体定向水解和生产。从培养的细菌和真菌中获得的酯酶具有多种工业应用。
酯酶是将酯分解为酸和醇的水解酶。
包装
无底玻璃瓶。内含物在插入的融合锥体内。
其他说明
1U相当于在 pH 7.5，30°C 下每分钟转化1 μmol 4-硝基苯基-L-乙酸盐的酶量。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Birgit Heinze et al.
Protein engineering, design & selection : PEDS, 20(3), 125-131 (2007-02-21)
Enzyme-catalyzed kinetic resolutions of secondary alcohols are a standard procedure today and several lipases and esterases have been described to show high activity and enantioselectivity. In contrast, tertiary alcohols and their esters are accepted only by a few biocatalysts. Only
Jessica Lusty Beech et al.
RSC advances, 12(13), 8119-8130 (2022-04-16)
Esterase enzymes catalyze diverse hydrolysis reactions with important biological, commercial, and biotechnological applications. For the improvement of these biocatalysts, there is a need for widely accessible, inexpensive, and adaptable activity screening assays that identify enzymes with particular substrate specificities. Natural
New citation. Highly Enantioselective Synthesis of Arylaliphatic Tertiary Alcohols using Mutants of an Esterase from Bacillus subtilis
Robert Kourist, Sebastian Bartsch, et al.
Advanced Synthesis & Catalysis, 349, 1393-1398 (2007)
Soil-based gene discovery: a new technology to accelerate and broaden biocatalytic applications
Gray K A, et al.
Advances in Applied Microbiology, 52, 1-28 (2003)
XieMei Tang et al.
Critical reviews in eukaryotic gene expression, 22(3), 179-187 (2012-11-13)
Tuberculosis remains one of the most prevalent and deadly infectious diseases, largely due to the emergence of multidrug-resistant and extensive drug-resistant Mycobacterium tuberculosis, especially the coinfection with HIV. Mycobacterium Ag85 complex (Ag85A, B, and C), with a carboxylesterase consensus sequence
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