产品线
BioReagent
质量水平
方案
≥90% (HPCE)
表单
powder
制造商/商品名称
ATTO-TEC GmbH
透射比
254 nm
647 nm
荧光
λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid)
紫外吸收
λ: 645-651 nm Amax
适用性
suitable for fluorescence
储存温度
−20°C
一般描述
Atto 647 是一种两性离子染料，净电荷为零。本品对 pH 敏感。虽然在 pH 8.5 时基本稳定，但在 pH 较高时缓慢降解。Atto 647 是一种新标记物，具有较高的分子吸收 (120,000) 和量子产率 (0.20)，并且在激发和发射最大值之间有足够的斯托克斯位移。Atto 647 具有高光稳定性和高亮度的特点。此外，该标记物水溶性好，三联体形成能力低。
应用
Atto 荧光标记专为高灵敏度应用设计，包括单分子检测。Atto 标记物具有刚性结构，不显示任何 顺式-反式异构化。因此，这些标记物表现出超常的强度，并且在共轭时具有极小的光谱移位。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Cytokinesis,Volume 137 of Methods in Cell Biology (2017)
Duncan Hill et al.
Biomedical microdevices, 13(4), 759-767 (2011-05-12)
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a
Laura Pastorino et al.
Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)
The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their
Dagmar Hildebrand et al.
Infection and immunity, 79(1), 220-228 (2010-10-20)
Pasteurella multocida is a Gram-negative bacillus that infects a number of wild and domestic animals, causing respiratory diseases. Toxigenic Pasteurella multocida strains produce a protein toxin (PMT) that leads to atrophic rhinitis in swine due to enhanced osteoclastogenesis and the
Emmanuel G Guignet et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 8(8), 1221-1227 (2007-04-17)
Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacetate fluorescent probes are reversibly bound to oligohistidine sequences
商品
荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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