T-3-CI-2-0.fl cell line

Study of erythroid differentiation

Mouse friend leukemia lymphoblast

T-3-Cl-2-0.fl is a clone of T-3-Cl-2, which originated from a splenic lesion of Friend virus-induced leukemia in a DD mouse. The cell line can be induced to differentiate into erythroblastoid cells with 1.5% DMSO. T-3-Cl-2-0.fl has been eradicated from mycoplasma using MC-210 by the originator.

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 3-9 x 100,000 cells/ml; 5%CO₂; 37ºC

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.