Human embryo kidney

The cell line HH-8 was generated by transfecting a vector (pcDNA3) containing a truncated calcium channel cDNA into (HEK) 293 cells (ECACC catalogue no. 85120602). The vector is constituitively expressed in the HH-8 cells producing a truncated (at amino acid 1733) cardiac α 1c-a calcium channel.

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS + 200 μg/ml Geneticin

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin, trypsin/EDTA, or PBS wash, 5% CO₂; 37°C. Cells may take up to 7 days to attach after resuscitation and sub culture. Cells grow in large aggregates that do not grow to confluency. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and re-seed to allow re-attachment of cells.

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RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.